Assessment of cytotoxicity, odontoblast-like differentiation, shear bond strength, and microhardness of four orthodontic adhesive composites.

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of oral science Pub Date : 2024-10-16 Epub Date: 2024-08-20 DOI:10.2334/josnusd.24-0061

David Bautista-Martinez, Rogelio J Scougall-Vilchis, Rosalía Contreras-Bulnes, Rene Garcia-Contreras

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引用次数: 0

Abstract

Purpose: To determine and compare the cytotoxicity, odontoblast-like differentiation, shear bond strength (SBS) and Vickers microhardness of four commercial light-cured orthodontic adhesives.

Methods: The orthodontic resins selected were Transbond XT - GI, Transbond Plus Color Change - GII (both from 3M Unitek), Enlight - GIII and Blugloo - GIV (both from Ormco). Samples were prepared, and leached monomers were obtained. Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and deposited calcium was analyzed using Alizarin red staining (ARS). SBS and the adhesive remnant index (ARI) were determined using 120 human premolars. The Vickers microhardness test was performed on the resin discs.

Results: All adhesives showed moderate to severe cytotoxicity (21-37%) and promoted similar formation of calcium deposits. A SBS of 6-8 MPa was achieved only by Blugloo (7.1 ± 2.4 MPa), and Enlight showed the lowest Vickers hardness score (40 ± 2.5 HV). Transbond Plus Color Change (score 0 = 42.9%) and Blugloo (score 0 = 46.4%) showed better ARI scores than Transbond XT (score 0 = 7.1%) and Enlight (score 0 = 3.6%).

Conclusion: On the basis of the properties evaluated, Blugloo seems to be the best option.

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评估四种正畸粘合剂复合材料的细胞毒性、牙母细胞样分化、剪切粘接强度和显微硬度。

目的：测定并比较四种商用光固化正畸粘合剂的细胞毒性、牙体母细胞样分化、剪切粘接强度（SBS）和维氏显微硬度：选择的正畸树脂是 Transbond XT - GI、Transbond Plus Color Change - GII（均来自 3M Unitek）、Enlight - GIII 和 Blugloo - GIV（均来自 Ormco）。制备样品并获得浸出单体。使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）法评估细胞毒性，使用茜素红染色法（ARS）分析沉积的钙。使用 120 颗人类前臼齿测定了 SBS 和粘附残余指数 (ARI)。对树脂盘进行了维氏硬度测试：结果：所有粘合剂都显示出中度到重度的细胞毒性（21%-37%），并促进形成类似的钙沉积。只有 Blugloo 的 SBS 达到了 6-8 MPa（7.1 ± 2.4 MPa），Enlight 的维氏硬度得分最低（40 ± 2.5 HV）。Transbond Plus Color Change（0 分 = 42.9%）和 Blugloo（0 分 = 46.4%）的 ARI 分数优于 Transbond XT（0 分 = 7.1%）和 Enlight（0 分 = 3.6%）：结论：根据所评估的特性，Blugloo 似乎是最佳选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral science DENTISTRY, ORAL SURGERY & MEDICINE-MATERIALS SCIENCE, BIOMATERIALS

CiteScore

3.80

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0.00%

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