Fabrication of Glucose Fluorescent Aptasensor Based on CdTe Quantum Dots.

Abstract

Diabetes is a chronic metabolic disease characterized by high blood glucose (or blood sugar) levels, which harms the heart, blood vessels, eyes, kidneys, and nerves over time. So, it is crucial to regularly control glucose concentration in biological fluids to check its targets, reduce unpleasant symptoms of high and low blood sugar, and avoid long-term diabetes complications. This study developed a simple, rapid, sensitive, and cost-effective fluorescence system for glucose determination. The fluorescent Aptasensor was fabricated using cadmium telluride quantum dots (CdTe QDs) modified with thioglycolic acid and functionalized with thiol-glucose-aptamer through ligand exchange. The thiol-glucose-aptamer interacted directly with CdTe QDs, increasing fluorescence intensity. However, it decreased when the target molecules of glucose were introduced. The structural and morphological characteristics of the Aptasensor were confirmed by various analytical methods such as UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), energy dispersive x-ray spectroscopy (EDX), transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). According to the typical Stern-Volmer equation, the relationship between fluorescent quenching and target concentration was linear with a detection limit (LOD) of 0.13 ± 1.95 × 10^-11 mol L^-1 and a relative standard deviation (RSD) of 1.05%. The Aptasensor demonstrated high specificity towards the target and stability over 28 days. Furthermore, it detected glucose in human serum and urine with a recovery rate of up to 99.74%. The results indicate that the fluorescent Aptasensor could be valuable in developing robust sensing technology for low-concentrated analytes.