Candida species-specific colonization in the healthy and impaired human gastrointestinal tract as simulated using the Mucosal Ileum-SHIME® model.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Benoît Marsaux, Frédéric Moens, Gies Vandevijver, Massimo Marzorati, Tom van de Wiele
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引用次数: 0

Abstract

Candida species primarily exist as harmless commensals in the gastrointestinal tract of warm-blooded animals. However, they can also cause life-threatening infections, which are often associated with gut microbial dysbiosis. Identifying the microbial actors that restrict Candida to commensalism remains a significant challenge. In vitro models could enable a mechanistic study of the interactions between Candida and simulated colon microbiomes. Therefore, this study aimed to elucidate the spatial and temporal colonization kinetics of specific Candida, including C. albicans, C. tropicalis, and C. parapsilosis, and their relative Nakaseomyces glabratus, by using an adapted SHIME® model, simulating the ileum, and proximal and distal colons. We monitored fungal and bacterial colonization kinetics under conditions of eubiosis (commensal lifestyle) and antibiotic-induced dysbiosis (pathogenic lifestyle). Our findings highlighted the variability in the colonization potential of Candida species across different intestinal regions. The ileum compartment proved to be the most favourable environment for C. albicans and C. parapsilosis under conditions of eubiosis. Antibiotic-induced dysbiosis resulted in resurgence of opportunistic Candida species, especially C. tropicalis and C. albicans. Future research should focus on identifying specific bacterial species influencing Candida colonization resistance and explore the long-term effects of antibiotics on the mycobiome and bacteriome.

使用 Mucosal Ileum-SHIME® 模型模拟健康和受损人体胃肠道中念珠菌物种特异性定植。
念珠菌主要作为无害的共生菌存在于温血动物的胃肠道中。然而,它们也能引起危及生命的感染,这通常与肠道微生物菌群失调有关。确定限制念珠菌共生的微生物角色仍然是一项重大挑战。体外模型可以对念珠菌与模拟结肠微生物群之间的相互作用进行机理研究。因此,本研究利用改良的 SHIME® 模型模拟回肠、近端和远端结肠,旨在阐明特定念珠菌(包括白念珠菌、热带念珠菌和副丝状念珠菌)及其亲缘种 Nakaseomyces glabratus 的空间和时间定植动力学。我们监测了真菌和细菌在优生(共生生活方式)和抗生素引起的菌群失调(致病生活方式)条件下的定植动力学。我们的研究结果表明,念珠菌在不同肠道区域的定植潜力存在差异。事实证明,在优生条件下,回肠是白念珠菌和副丝状念珠菌最有利的生长环境。抗生素引起的菌群失调导致机会性念珠菌,尤其是热带念珠菌和白念珠菌的重新出现。未来的研究应侧重于确定影响念珠菌定植耐药性的特定细菌种类,并探索抗生素对真菌生物群和细菌群的长期影响。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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