High Performance Liquid chromatography - Fourier Transform Infrared Spectroscopy Coupling: A Comprehensive Review.

IF 4.2 2区化学 Q1 CHEMISTRY, ANALYTICAL

Critical reviews in analytical chemistry Pub Date : 2024-08-21 DOI:10.1080/10408347.2024.2391892

Radoslav Halko, Denis Pavelek, Massoud Kaykhaii

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Abstract

This review presents a critical examination of the interface for coupling high performance liquid chromatography (HPLC) with Fourier transform infrared spectrometry (FTIR) since 2010. This coupling offers a robust analytical approach characterized by exceptional chemical specificity and the capacity to analyze complex multi-component mixtures qualitatively and quantitatively with high sensitivity, particularly in low limit of detection ranges. This coupling enables the identification of individual components of a mixture by IR after their separation by HPLC, although challenges arise from the potential distortion of infrared spectra by mobile phase components. Addressing this issue necessitates the implementation of suitable interfaces, such as flow cells or off-line indirect measurement methods like hot inert gas streams or ultrasonic nebulizers. The key parameters influencing the coupling of HPLC-FTIR include the solvent elimination methods, mode of FTIR technique, and IR background for accurate analyte identification. Moreover, the composition of the mobile phase and the utilization of buffer solutions in the HPLC mobile phase profoundly impact analyte identification by FTIR.

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高效液相色谱-傅立叶变换红外光谱耦合：全面回顾。

本综述对 2010 年以来将高效液相色谱法（HPLC）与傅立叶变换红外光谱法（FTIR）联用的界面进行了深入研究。这种耦合提供了一种稳健的分析方法，其特点是化学特异性极强，能够对复杂的多组分混合物进行高灵敏度的定性和定量分析，特别是在低检测限范围内。这种耦合方法可以在使用高效液相色谱法分离混合物中的单个成分后，通过红外光谱对其进行鉴定，但流动相成分可能会导致红外光谱失真。要解决这一问题，就必须采用合适的接口，如流动池或离线间接测量方法，如热惰性气体流或超声雾化器。影响 HPLC-FTIR 联用的关键参数包括溶剂消除方法、FTIR 技术模式以及准确鉴定分析物的红外背景。此外，HPLC 流动相中的流动相成分和缓冲溶液的使用也会对傅立叶变换红外光谱的分析鉴定产生深远影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical reviews in analytical chemistry 化学-分析化学

CiteScore

12.00

自引率

4.00%

发文量

137

审稿时长

6 months

期刊介绍： Critical Reviews in Analytical Chemistry continues to be a dependable resource for both the expert and the student by providing in-depth, scholarly, insightful reviews of important topics within the discipline of analytical chemistry and related measurement sciences. The journal exclusively publishes review articles that illuminate the underlying science, that evaluate the field''s status by putting recent developments into proper perspective and context, and that speculate on possible future developments. A limited number of articles are of a "tutorial" format written by experts for scientists seeking introduction or clarification in a new area. This journal serves as a forum for linking various underlying components in broad and interdisciplinary means, while maintaining balance between applied and fundamental research. Topics we are interested in receiving reviews on are the following: · chemical analysis; · instrumentation; · chemometrics; · analytical biochemistry; · medicinal analysis; · forensics; · environmental sciences; · applied physics; · and material science.

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