Dysregulation of Aquaporin-3 and Glyceryl Glucoside Restoring Action in Hidradenitis Suppurativa in Vitro Models.

IF 2.5 Q3 CELL BIOLOGY
Cecilia Del Vecchio, Rossella Gratton, Cécile Nait-Meddour, Elena Maria Nardacchione, Ronald Moura, Eduardo Sommella, Chiara Moltrasio, Angelo Valerio Marzano, Blendi Ura, Donatella Mentino, Michele Boniotto, Adamo Pio d'Adamo, Giuseppe Calamita, Sergio Crovella, Paola Maura Tricarico
{"title":"Dysregulation of Aquaporin-3 and Glyceryl Glucoside Restoring Action in Hidradenitis Suppurativa in Vitro Models.","authors":"Cecilia Del Vecchio, Rossella Gratton, Cécile Nait-Meddour, Elena Maria Nardacchione, Ronald Moura, Eduardo Sommella, Chiara Moltrasio, Angelo Valerio Marzano, Blendi Ura, Donatella Mentino, Michele Boniotto, Adamo Pio d'Adamo, Giuseppe Calamita, Sergio Crovella, Paola Maura Tricarico","doi":"10.33594/000000720","DOIUrl":null,"url":null,"abstract":"<p><strong>Background/aims: </strong>Aquaporin-3 (AQP3) is an aquaglyceroporin and peroxiporin that plays a crucial role in skin barrier homeostasis. Dysregulated AQP3 expression has been observed in different inflammatory skin conditions. Hidradenitis Suppurativa (HS) is an autoinflammatory keratinization disease that typically appears between 10 and 21 years of age, characterized by alteration of skin barrier homeostasis.</p><p><strong>Methods: </strong>To evaluate <i>in vitro</i> the role of AQP3 in the development of HS, we performed real-time PCR and Western blot to analyze gene and protein levels in human keratinocyte cell lines knock-out (KO) for <i>NCSTN</i> and <i>PSENEN</i> genes, simulating genetic-associated HS. Additionally, we investigated the impact of Glyceryl Glucoside (GG) on biological processes by performing MTT, scratch, proliferation assays and proteome studies.</p><p><strong>Results: </strong>We detected a significant decrease of the levels of AQP3 gene and protein in KO cell lines. GG effectively elevated the levels of mRNA and protein, significantly decreased the hyperproliferation rate, and enhanced cell migration in our <i>in vitro</i> model of genetic Hidradenitis Suppurativa. Pathway enrichment analysis further confirmed GG's role in the migration and proliferation pathways of keratinocytes.</p><p><strong>Conclusion: </strong>Our results suggest that AQP3 may act as a new novel actor in HS etio-pathogenesis, and GG could be further explored as potential treatment option for managing HS in patients.</p>","PeriodicalId":9845,"journal":{"name":"Cellular Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular Physiology and Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33594/000000720","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background/aims: Aquaporin-3 (AQP3) is an aquaglyceroporin and peroxiporin that plays a crucial role in skin barrier homeostasis. Dysregulated AQP3 expression has been observed in different inflammatory skin conditions. Hidradenitis Suppurativa (HS) is an autoinflammatory keratinization disease that typically appears between 10 and 21 years of age, characterized by alteration of skin barrier homeostasis.

Methods: To evaluate in vitro the role of AQP3 in the development of HS, we performed real-time PCR and Western blot to analyze gene and protein levels in human keratinocyte cell lines knock-out (KO) for NCSTN and PSENEN genes, simulating genetic-associated HS. Additionally, we investigated the impact of Glyceryl Glucoside (GG) on biological processes by performing MTT, scratch, proliferation assays and proteome studies.

Results: We detected a significant decrease of the levels of AQP3 gene and protein in KO cell lines. GG effectively elevated the levels of mRNA and protein, significantly decreased the hyperproliferation rate, and enhanced cell migration in our in vitro model of genetic Hidradenitis Suppurativa. Pathway enrichment analysis further confirmed GG's role in the migration and proliferation pathways of keratinocytes.

Conclusion: Our results suggest that AQP3 may act as a new novel actor in HS etio-pathogenesis, and GG could be further explored as potential treatment option for managing HS in patients.

体外模型中 Aquaporin-3 的失调与甘油葡萄糖苷的恢复作用
背景/目的:水通道蛋白-3(AQP3)是一种水甘油和过氧脂蛋白,在皮肤屏障稳态中起着至关重要的作用。在不同的炎症性皮肤病中均可观察到 AQP3 表达失调。湿疹(HS)是一种自身炎症性角质化疾病,通常出现在 10 至 21 岁之间,其特点是皮肤屏障平衡的改变:为了在体外评估AQP3在HS发病过程中的作用,我们在敲除(KO)NCSTN和PSENEN基因的人角质细胞系中进行了实时PCR和Western印迹,分析基因和蛋白水平,模拟遗传相关的HS。此外,我们还通过 MTT、划痕、增殖试验和蛋白质组研究,探讨了甘油葡萄糖苷(GG)对生物过程的影响:结果:我们在 KO 细胞系中检测到 AQP3 基因和蛋白水平明显下降。在遗传性扁平湿疹的体外模型中,GG 能有效提高 mRNA 和蛋白质的水平,显著降低细胞的过度增殖率,并增强细胞的迁移能力。通路富集分析进一步证实了 GG 在角质形成细胞迁移和增殖通路中的作用:我们的研究结果表明,AQP3可能是影响HS发病机制的一个新角色,GG可作为治疗HS患者的潜在治疗方案进行进一步探索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信