Impact of Organochlorine Pesticides Exposure on Histone Modification H3K9ac: Implications for Unexplained Recurrent Miscarriage.

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Sanaz Faramarz, Gholamreza Asadikaram, Mojtaba Abbasi-Jorjandi, Moslem Abolhassani, Katayoun Alidousti, Parvin Mangolian Shahrbabaki, Hossein Pourghadamyari
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引用次数: 0

Abstract

Epigenetic alterations, changes in gene expression without DNA sequence modifications, are associated with various health disorders, including reproductive health issues. These alterations can be influenced by environmental factors such as pesticides. This study aimed to explore the relationship between exposure to Organochlorine Pesticides (OClPs) and the histone modification mark H3K9ac in the placenta and fetal tissue, in the context of unexplained recurrent miscarriage (URM). In the case-control study, serum samples from 73 women with URM and 30 healthy women were examined for the presence of OClPs, which include 2,4-DDT, 2,4-DDE, 4,4-DDT, 4,4-DDE, α-HCH, β-HCH, and γ-HCH, using gas chromatography. Western blot analysis was used to assess H3K9ac expression in placental and fetal tissues. In the URM group, significant increases were observed in the values of α-HCH, β-HCH, 2,4-DDE, and 4,4-DDE, as well as in the concentration of total OClPs (Ʃ3HCH, Ʃ2DDE, Ʃ2DDT, and Ʃ7OClP), compared to controls. While H3K9ac levels in fetal tissue showed no significant difference, a notable decrease was found in the placental tissue of the URM. In the placenta tissue of URM, logistic regression analysis also revealed a significant inverse correlation between the toxins α-HCH, 2,4-DDE, 4,4-DDE, 4,4-DDT, total OClPs, and reduced H3K9ac expression. Our findings suggest that OClPs exposure may contribute to URM by reducing H3K9ac expression in the placenta, potentially affecting placental growth and immune tolerance. This underscores the need for further investigation into the involved mechanisms and potential therapeutic interventions, and the importance of OClPs regulation for reproductive health protection.

Abstract Image

有机氯农药暴露对组蛋白修饰 H3K9ac 的影响:不明原因的复发性流产的影响。
表观遗传学改变,即基因表达的改变而不改变 DNA 序列,与包括生殖健康问题在内的各种健康疾病有关。这些改变可能受到农药等环境因素的影响。本研究旨在探讨不明原因复发性流产(URM)中,有机氯农药(OClPs)暴露与胎盘和胎儿组织中组蛋白修饰标记 H3K9ac 之间的关系。在这项病例对照研究中,研究人员利用气相色谱法检测了73名患复发性流产的妇女和30名健康妇女的血清样本中是否含有OClPs,其中包括2,4-DDT、2,4-DDE、4,4-DDT、4,4-DDE、α-HCH、β-HCH和γ-HCH。采用 Western 印迹分析评估 H3K9ac 在胎盘和胎儿组织中的表达。在 URM 组中,与对照组相比,α-HCH、β-HCH、2,4-DDE 和 4,4-DDE,以及总 OClPs(Ʃ3HCH、Ʃ2DDE、Ʃ2DDT 和 Ʃ7OClP)的浓度都明显增加。虽然胎儿组织中的 H3K9ac 水平没有明显差异,但尿毒症患者的胎盘组织中的 H3K9ac 水平却明显下降。在尿毒症患者的胎盘组织中,逻辑回归分析还发现α-HCH、2,4-DDE、4,4-DDE、4,4-DDT、OClPs总量与H3K9ac表达量减少之间存在显著的反相关关系。我们的研究结果表明,接触 OClPs 可能会降低胎盘中 H3K9ac 的表达,从而导致尿毒症,并可能影响胎盘生长和免疫耐受。这强调了进一步研究相关机制和潜在治疗干预措施的必要性,以及调控 OClPs 对保护生殖健康的重要性。
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来源期刊
Biochemical Genetics
Biochemical Genetics 生物-生化与分子生物学
CiteScore
3.90
自引率
0.00%
发文量
133
审稿时长
4.8 months
期刊介绍: Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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