{"title":"Surveillance of influenza A and B viruses from community and hospital wastewater treatment plants","authors":"Sneka Panneerselvam, Athira Manayan Parambil, Anup Jayaram, Prasad Varamballi, Chiranjay Mukhopadhyay, Anitha Jagadesh","doi":"10.1111/1758-2229.13317","DOIUrl":null,"url":null,"abstract":"<p>Influenza virus is a well-known pathogen that can cause epidemics and pandemics. Several surveillance methods are being followed to monitor the transmission patterns and spread of influenza in the community. Wastewater-based Epidemiology (WBE) can serve as an additional tool to detect the presence of influenza viruses. The current study primarily focuses on surveillance of Influenza A and Influenza B in wastewater treatment plant (WWTP) samples. A total of 100 wastewater samples were collected in July (<i>n</i> = 50) and August (<i>n</i> = 50) 2023 from four different WWTPs in Manipal and Udupi, district of Karnataka, India. The WWTP samples were processed and tested by Real-Time reverse transcriptase PCR (RT-PCR). The data generated was analysed in comparison with the clinical Influenza cases. Of the 100 samples, 18 (18%) tested positive for Influenza A virus and 2 (2%) tested positive for Influenza B virus, with a viral load ranging 1.4 x 10<sup>2</sup>–2.2 x 10<sup>3</sup> gc/L for influenza A virus and 5.2 x 10<sup>3</sup>–7.7 x 10<sup>3</sup>gc/L for influenza B virus. On correlating the WWTP positivity with clinical case, it was found that influenza clinical cases and virus positivity in wastewater increased simultaneously, emphasizing WBE as a concurrent method for monitoring influenza virus activity.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 4","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.13317","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.13317","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Influenza virus is a well-known pathogen that can cause epidemics and pandemics. Several surveillance methods are being followed to monitor the transmission patterns and spread of influenza in the community. Wastewater-based Epidemiology (WBE) can serve as an additional tool to detect the presence of influenza viruses. The current study primarily focuses on surveillance of Influenza A and Influenza B in wastewater treatment plant (WWTP) samples. A total of 100 wastewater samples were collected in July (n = 50) and August (n = 50) 2023 from four different WWTPs in Manipal and Udupi, district of Karnataka, India. The WWTP samples were processed and tested by Real-Time reverse transcriptase PCR (RT-PCR). The data generated was analysed in comparison with the clinical Influenza cases. Of the 100 samples, 18 (18%) tested positive for Influenza A virus and 2 (2%) tested positive for Influenza B virus, with a viral load ranging 1.4 x 102–2.2 x 103 gc/L for influenza A virus and 5.2 x 103–7.7 x 103gc/L for influenza B virus. On correlating the WWTP positivity with clinical case, it was found that influenza clinical cases and virus positivity in wastewater increased simultaneously, emphasizing WBE as a concurrent method for monitoring influenza virus activity.
期刊介绍:
The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side.
Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following:
the structure, activities and communal behaviour of microbial communities
microbial community genetics and evolutionary processes
microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors
microbes in the tree of life, microbial diversification and evolution
population biology and clonal structure
microbial metabolic and structural diversity
microbial physiology, growth and survival
microbes and surfaces, adhesion and biofouling
responses to environmental signals and stress factors
modelling and theory development
pollution microbiology
extremophiles and life in extreme and unusual little-explored habitats
element cycles and biogeochemical processes, primary and secondary production
microbes in a changing world, microbially-influenced global changes
evolution and diversity of archaeal and bacterial viruses
new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.