Influence of growth medium on the species-specific interactions between algae and bacteria

Abstract

In this study, we investigated a species-specific algal–bacterial co-culture that has recently attracted worldwide scientific attention as a novel approach to enhancing algal growth rate. We report that the type of interaction between Chlorella vulgaris and bacteria of the genus Delftia is not solely determined by species specificity. Rather, it is a dynamic process of adaptation to the surrounding conditions, where one or the other microorganism dominates (temporally) depending on the growth conditions, in particular the medium. Under laboratory conditions, we found that Delftia sp. had a negative effect on C. vulgaris growth when co-cultured in a TAP medium. However, the co-culture of algae and bacteria under BG-11 and BG-11 + acetic acid resulted in an increase in algal concentration compared to algal cultures without bacteria under the same conditions. Additional chemical analysis revealed that the presence of different carbon (the main organic carbon source—acetic acid in TAP or BG-11 + acetic acid medium and inorganic carbon source—Na₂CO₃ in BG-11 or BG-11 + acetic acid medium) and nitrogen (NH₄Cl in TAP medium and NaNO₃ in BG-11 or BG-11 + acetic acid medium) species in the growth medium was one of the main factors driving the shift in interaction type.