The OsAGO2–OsNAC300–OsNAP module regulates leaf senescence in rice

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Shaoyan Zheng, Junyu Chen, Ying He, Jingqin Lu, Hong Chen, Zipeng Liang, Junqi Zhang, Zhenlan Liu, Jing Li, Chuxiong Zhuang
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Abstract

Leaves play a crucial role in the growth and development of rice (Oryza sativa) as sites for the production of photosynthesis. Early leaf senescence leads to substantial drops in rice yields. Whether and how DNA methylation regulates gene expression and affects leaf senescence remains elusive. Here, we demonstrate that mutations in rice ARGONAUTE 2 (OsAGO2) lead to premature leaf senescence, with chloroplasts in Osago2 having lower chlorophyll content and an abnormal thylakoid structure compared with those from wild-type plants. We show that OsAGO2 associates with a 24-nt microRNA and binds to the promoter region of OsNAC300, which causes DNA methylation and suppressed expression of OsNAC300. Overexpressing OsNAC300 causes the similar premature leaf senescence as Osago2 mutants and knocking out OsNAC300 in the Osago2 mutant background suppresses the early senescence of Osago2 mutants. Based on yeast one-hybrid, dual-luciferase, and electrophoresis mobility shift assays, we propose that OsNAC300 directly regulates transcription of the key rice aging gene NAC-like, activated by APETALA3/PISTILLATA (OsNAP) to control leaf senescence. Our results unravel a previously unknown epigenetic regulatory mechanism underlying leaf senescence in which OsAGO2–OsNAC300–OsNAP acts as a key regulatory module of leaf senescence to maintain leaf function.

Abstract Image

OsAGO2-OsNAC300-OsNAP 模块调控水稻叶片的衰老。
叶片作为光合作用的场所,在水稻(Oryza sativa)的生长发育过程中起着至关重要的作用。早期叶片衰老会导致水稻产量大幅下降。DNA 甲基化是否以及如何调控基因表达并影响叶片衰老仍是一个未知数。在这里,我们证明了水稻 ARGONAUTE 2(OsAGO2)的突变会导致叶片过早衰老,与野生型植株相比,Osago2 的叶绿体叶绿素含量较低,类木质结构异常。我们发现 OsAGO2 与 24-nt microRNA 结合,并与 OsNAC300 的启动子区域结合,从而导致 DNA 甲基化,抑制 OsNAC300 的表达。过量表达OsNAC300会导致与Osago2突变体相似的叶片过早衰老,而在Osago2突变体背景下敲除OsNAC300会抑制Osago2突变体的早期衰老。基于酵母单杂交、双荧光素酶和电泳迁移实验,我们提出 OsNAC300 直接调控关键水稻衰老基因 NAC-like 的转录,由 APETALA3/PISTILLATA (OsNAP) 激活,从而控制叶片衰老。我们的研究结果揭示了一种之前未知的叶片衰老的表观遗传调控机制,其中 OsAGO2-OsNAC300-OsNAP 作为叶片衰老的一个关键调控模块维持叶片功能。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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