Impact of temperature and water source on drinking water microbiome during distribution in a pilot-scale study

IF 10.4 1区 工程技术 Q1 ENGINEERING, CHEMICAL
Fien Waegenaar, Thomas Pluym, Laura Coene, Jozefien Schelfhout, Cristina García-Timermans, Bart De Gusseme, Nico Boon
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Abstract

This study utilized a pilot-scale distribution network to examine the impact of temperature increases (16 °C, 20 °C, 24 °C) and source variations (treated ground- and surface water) on bulk and biofilm communities over 137 days. Microbial characterization employed flow cytometry and 16 S rRNA gene-based amplicon sequencing to elucidate bulk-biofilm interactions. Bacterial bulk cell densities increased with higher temperatures, while water source variations significantly influenced bulk cell densities as well as the community composition. Additionally, growth curves were fitted on the flow cytometry results, and growth rates and carrying capacities were higher with treated groundwater at elevated temperatures. Conversely, biofilm cell densities remained unaffected by temperature. A mature biofilm was observed from day 70 onwards and a core biofilm microbiome, resilient to temperature and water source changes, was identified. These findings emphasize the importance of water source quality for maintaining biological stability in drinking water systems, particularly in the face of changing environmental conditions.

Abstract Image

在一项试点研究中,温度和水源对饮用水分配过程中微生物群的影响
本研究利用一个试点规模的分配网络,在 137 天内考察了温度升高(16 °C、20 °C、24 °C)和水源变化(处理过的地下水和地表水)对散装水和生物膜群落的影响。微生物特征描述采用了流式细胞仪和基于 16 S rRNA 基因的扩增子测序技术,以阐明体膜与生物膜之间的相互作用。细菌体细胞密度随温度升高而增加,而水源的变化对体细胞密度和群落组成有显著影响。此外,根据流式细胞仪的结果拟合了生长曲线,在温度升高时,经过处理的地下水的生长率和承载能力更高。相反,生物膜细胞密度不受温度影响。从第 70 天起,就能观察到成熟的生物膜,并确定了核心生物膜微生物群,它们对温度和水源的变化具有抵抗力。这些发现强调了水源质量对保持饮用水系统生物稳定性的重要性,尤其是在环境条件不断变化的情况下。
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来源期刊
npj Clean Water
npj Clean Water Environmental Science-Water Science and Technology
CiteScore
15.30
自引率
2.60%
发文量
61
审稿时长
5 weeks
期刊介绍: npj Clean Water publishes high-quality papers that report cutting-edge science, technology, applications, policies, and societal issues contributing to a more sustainable supply of clean water. The journal's publications may also support and accelerate the achievement of Sustainable Development Goal 6, which focuses on clean water and sanitation.
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