A novel live DNA tagging system for African swine fever virus shows that bisbenzimide Hoechst 33342 can effectively block its replication

IF 4.5 2区医学 Q1 PHARMACOLOGY & PHARMACY

Antiviral research Pub Date : 2024-08-19 DOI:10.1016/j.antiviral.2024.105973

Veronica Martin , Beatriz Guerra , Bruno Hernaez , Sandrine Kappler-Gratias , Franck Gallardo , Milagros Guerra , German Andres , Ali Alejo

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引用次数: 0

Abstract

African swine fever virus (ASFV) infection causes a frequently fatal disease in domestic swine that has affected more than 50 countries worldwide since 2021, with a major impact on animal welfare and economy. The development of effective vaccines or antivirals against this disease are urgently required for its effective control. Live detection of viral replication has been used as a tool for the screening and characterization of antiviral compounds in other dsDNA genome containing viruses. Here, we have adapted the ANCHOR fluorescent DNA labelling system to ASFV by constructing and characterizing a novel recombinant virus. We show that this virus is viable and effectively tags viral DNA replication sites, which can be detected and quantified in real time. Further, we have used high content cell microscopy to test the antiviral activity of bisbenzimide compounds and show that Hoechst 33342 has specific anti-ASFV activity. We expect this novel tool to be useful both in the further study of ASFV replication as in the screening of new specific antiviral compounds.

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一种新型非洲猪瘟病毒活 DNA 标记系统显示，双苯亚胺 Hoechst 33342 能有效阻断非洲猪瘟病毒的复制

非洲猪瘟病毒（ASFV）感染导致家猪经常患上致命疾病，自 2021 年以来已波及全球 50 多个国家，对动物福利和经济造成了重大影响。为有效控制该疾病，迫切需要开发有效的疫苗或抗病毒药物。病毒复制的活体检测已被用作筛选和鉴定其他含 dsDNA 基因组病毒的抗病毒化合物的工具。在这里，我们通过构建和鉴定一种新型重组病毒，将 ANCHOR 荧光 DNA 标记系统应用于 ASFV。我们的研究表明，这种病毒具有生命力，能有效标记病毒 DNA 复制位点，并能对其进行实时检测和定量。此外，我们还利用高含量细胞显微镜检测了双苯并咪唑类化合物的抗病毒活性，结果表明 Hoechst 33342 具有特异性抗 ASFV 活性。我们希望这种新工具能在进一步研究 ASFV 复制和筛选新的特异性抗病毒化合物方面发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antiviral research 医学-病毒学

CiteScore

17.10

自引率

3.90%

发文量

157

审稿时长

34 days

期刊介绍： Antiviral Research is a journal that focuses on various aspects of controlling viral infections in both humans and animals. It is a platform for publishing research reports, short communications, review articles, and commentaries. The journal covers a wide range of topics including antiviral drugs, antibodies, and host-response modifiers. These topics encompass their synthesis, in vitro and in vivo testing, as well as mechanisms of action. Additionally, the journal also publishes studies on the development of new or improved vaccines against viral infections in humans. It delves into assessing the safety of drugs and vaccines, tracking the evolution of drug or vaccine-resistant viruses, and developing effective countermeasures. Another area of interest includes the identification and validation of new drug targets. The journal further explores laboratory animal models of viral diseases, investigates the pathogenesis of viral diseases, and examines the mechanisms by which viruses avoid host immune responses.