Light-Responsive and Antibacterial Graphenic Materials as a Holistic Approach to Tissue Engineering

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY
Andrea Ferreras, Ana Matesanz, Jabier Mendizabal, Koldo Artola, Yuta Nishina, Pablo Acedo, José L. Jorcano, Amalia Ruiz*, Giacomo Reina* and Cristina Martín*, 
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引用次数: 0

Abstract

While the continuous development of advanced bioprinting technologies is under fervent study, enhancing the regenerative potential of hydrogel-based constructs using external stimuli for wound dressing has yet to be tackled. Fibroblasts play a significant role in wound healing and tissue implants at different stages, including extracellular matrix production, collagen synthesis, and wound and tissue remodeling. This study explores the synergistic interplay between photothermal activity and nanomaterial-mediated cell proliferation. The use of different graphene-based materials (GBM) in the development of photoactive bioinks is investigated. In particular, we report the creation of a skin-inspired dressing for wound healing and regenerative medicine. Three distinct GBM, namely, graphene oxide (GO), reduced graphene oxide (rGO), and graphene platelets (GP), were rigorously characterized, and their photothermal capabilities were elucidated. Our investigations revealed that rGO exhibited the highest photothermal efficiency and antibacterial properties when irradiated, even at a concentration as low as 0.05 mg/mL, without compromising human fibroblast viability. Alginate-based bioinks alongside human fibroblasts were employed for the bioprinting with rGO. The scaffold did not affect the survival of fibroblasts for 3 days after bioprinting, as cell viability was not affected. Remarkably, the inclusion of rGO did not compromise the printability of the hydrogel, ensuring the successful fabrication of complex constructs. Furthermore, the presence of rGO in the final scaffold continued to provide the benefits of photothermal antimicrobial therapy without detrimentally affecting fibroblast growth. This outcome underscores the potential of rGO-enhanced hydrogels in tissue engineering and regenerative medicine applications. Our findings hold promise for developing game-changer strategies in 4D bioprinting to create smart and functional tissue constructs with high fibroblast proliferation and promising therapeutic capabilities in drug delivery and bactericidal skin-inspired dressings.

Abstract Image

光响应和抗菌石墨烯材料是组织工程的一种综合方法
虽然先进的生物打印技术正在不断发展,但利用外部刺激增强水凝胶基构建体的再生潜力以用于伤口包扎的问题仍有待解决。成纤维细胞在伤口愈合和组织植入的不同阶段发挥着重要作用,包括细胞外基质的产生、胶原蛋白的合成以及伤口和组织的重塑。本研究探讨了光热活性与纳米材料介导的细胞增殖之间的协同作用。我们研究了不同石墨烯基材料(GBM)在光活性生物墨水开发中的应用。特别是,我们报告了用于伤口愈合和再生医学的皮肤启发敷料的创造。我们对三种不同的 GBM,即氧化石墨烯(GO)、还原氧化石墨烯(rGO)和小石墨烯(GP)进行了严格的表征,并阐明了它们的光热功能。我们的研究发现,即使在浓度低至 0.05 毫克/毫升的情况下,rGO 在照射时也能表现出最高的光热效率和抗菌特性,同时不会影响人类成纤维细胞的活力。在使用 rGO 进行生物打印时,使用了海藻酸盐生物材料和人类成纤维细胞。在生物打印后的 3 天内,该支架不会影响成纤维细胞的存活,因为细胞活力未受影响。值得注意的是,rGO 的加入并没有影响水凝胶的可印刷性,从而确保了复杂构造的成功制造。此外,最终支架中的 rGO 还能继续提供光热抗菌治疗的益处,而不会对成纤维细胞的生长产生不利影响。这一结果凸显了 rGO 增强水凝胶在组织工程和再生医学应用中的潜力。我们的研究结果为开发改变游戏规则的 4D 生物打印战略带来了希望,从而创造出具有高成纤维细胞增殖能力的智能功能性组织结构,并在药物输送和皮肤杀菌敷料方面具有良好的治疗能力。
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来源期刊
ACS Nanoscience Au
ACS Nanoscience Au 材料科学、纳米科学-
CiteScore
4.20
自引率
0.00%
发文量
0
期刊介绍: ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
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