Evaluation of Ethanolic Extract of Prosopis Farcta and Trehalose on Cryopreserved Goat Epididymal Spermatozoa.

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-08-21 DOI:10.1089/bio.2023.0132

Saadoon Homar Ali, Abbas Farshad, Asaad Vaziry, Jalal Rostamzadeh, Farshad Ariyan

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引用次数: 0

Abstract

The purpose of this study, carried out in two experiments, was to investigate the antioxidant effect of Prosopis farcta in Experiment 1, and trehalose in Experiment 2 in the freezing extender, on the quality of frozen-thawed goat epididymal spermatozoa. Sperm samples were added to based egg-yolk Tris-extender containing experimental treatments. The first experimental treatments included the following: an extender of the control group without additive and extender containing 50, 100, or 150 µg/mL of Prosopis farcta ethanolic extract (PEE1, PEE2, and PEE3, respectively). Treatments of the second experiment include an extender of the control group without additive, an extender containing 100 mM of trehalose (Tr), an extender containing 100 µg/mL PEE2, and 100 µg/mL PEE2 + 100 mM Tr. The results of the first experiment showed that PEE2 compared with the control group led to a significant decrease (p < 0.05) in malondialdehyde (MDA) concentration. Also, PEE1 and PEE2 treatments resulted in a significant increase (p < 0.05) in motility parameters by computer-assisted sperm analysis, and MDA concentrations decreased significantly (p < 0.05) in all treatments compared with the control group. In general, the results of the present experiment showed that Prosopis farcta ethanolic extract at the level of 100 µg/mL was effective in improving the quality of frozen-thawed goat epididymal spermatozoa. Also, a combination of Prosopis farcta ethanolic extract and trehalose can be successful in freezing goat epididymal spermatozoa.

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远志的乙醇提取物和三卤糖对低温保存的山羊附睾精子的影响评估

本研究分两个实验进行，目的是研究实验 1 中的远志和实验 2 中的曲阿糖对冷冻解冻的山羊附睾精子质量的抗氧化作用。精子样本被添加到含有实验处理的基于蛋黄的 Tris 扩展剂中。第一种实验处理包括：不含添加剂的对照组扩展剂，以及分别含有 50、100 或 150 µg/mL 的远志乙醇提取物（PEE1、PEE2 和 PEE3）的扩展剂。第二次实验的处理包括对照组不含添加剂的扩展剂、含 100 mM 曲哈糖（Tr）的扩展剂、含 100 µg/mL PEE2 的扩展剂和 100 µg/mL PEE2 + 100 mM Tr。第一个实验的结果表明，与对照组相比，PEE2 使丙二醛（MDA）浓度显著下降（p < 0.05）。此外，通过计算机辅助精子分析，PEE1 和 PEE2 处理使精子活力参数显著增加（p < 0.05），与对照组相比，所有处理的 MDA 浓度均显著降低（p < 0.05）。总之，本实验的结果表明，100 µg/mL 的远志红乙醇提取物能有效改善冷冻解冻山羊附睾精子的质量。此外，远志乙醇提取物与妥尔糖的组合也能成功冷冻山羊附睾精子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

自引率

12.50%

发文量

114

期刊介绍： Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.