Single-cell RNA sequencing reveals the effects of high-fat diet on oocyte and early embryo development in female mice.

IF 4.2 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM
Qi Zhu, Feng Li, Hao Wang, Xia Wang, Yu Xiang, Huimin Ding, Honghui Wu, Cen Xu, Linglin Weng, Jieyu Cai, Tianyue Xu, Na Liang, Xiaoqi Hong, Mingrui Xue, Hongshan Ge
{"title":"Single-cell RNA sequencing reveals the effects of high-fat diet on oocyte and early embryo development in female mice.","authors":"Qi Zhu, Feng Li, Hao Wang, Xia Wang, Yu Xiang, Huimin Ding, Honghui Wu, Cen Xu, Linglin Weng, Jieyu Cai, Tianyue Xu, Na Liang, Xiaoqi Hong, Mingrui Xue, Hongshan Ge","doi":"10.1186/s12958-024-01279-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Obesity is a global health issue with detrimental effects on various human organs, including the reproductive system. Observational human data and several lines of animal experimental data suggest that maternal obesity impairs ovarian function and early embryo development, but the precise pathogenesis remains unclear.</p><p><strong>Methods: </strong>We established a high-fat diet (HFD)-induced obese female mouse model to assess systemic metabolism, ovarian morphology, and oocyte function in mice. For the first time, this study employed single-cell RNA sequencing to explore the altered transcriptomic landscape of preimplantation embryos at different stages in HFD-induced obese mice. Differential gene expression analysis, enrichment analysis and protein-protein interactions network analysis were performed.</p><p><strong>Results: </strong>HFD-induced obese female mice exhibited impaired glucolipid metabolism and insulin resistance. The ovaries of HFD mice had a reduced total follicle number, an increased proportion of atretic follicles, and irregular granulosa cell arrangement. Furthermore, the maturation rate of embryonic development by in vitro fertilization of oocytes was significantly decreased in HFD mice. Additionally, the transcriptional landscapes of preimplantation embryos at different stages in mice induced by different diets were significantly distinguished. The maternal-to-zygotic transition was also affected by the failure to remove maternal RNAs and to turn off zygotic genome expression.</p><p><strong>Conclusions: </strong>HFD-induced obesity impaired ovarian morphology and oocyte function in female mice and further led to alterations in the transcriptional landscape of preimplantation embryos at different stages of HFD mice.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"105"},"PeriodicalIF":4.2000,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334609/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive Biology and Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12958-024-01279-7","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Obesity is a global health issue with detrimental effects on various human organs, including the reproductive system. Observational human data and several lines of animal experimental data suggest that maternal obesity impairs ovarian function and early embryo development, but the precise pathogenesis remains unclear.

Methods: We established a high-fat diet (HFD)-induced obese female mouse model to assess systemic metabolism, ovarian morphology, and oocyte function in mice. For the first time, this study employed single-cell RNA sequencing to explore the altered transcriptomic landscape of preimplantation embryos at different stages in HFD-induced obese mice. Differential gene expression analysis, enrichment analysis and protein-protein interactions network analysis were performed.

Results: HFD-induced obese female mice exhibited impaired glucolipid metabolism and insulin resistance. The ovaries of HFD mice had a reduced total follicle number, an increased proportion of atretic follicles, and irregular granulosa cell arrangement. Furthermore, the maturation rate of embryonic development by in vitro fertilization of oocytes was significantly decreased in HFD mice. Additionally, the transcriptional landscapes of preimplantation embryos at different stages in mice induced by different diets were significantly distinguished. The maternal-to-zygotic transition was also affected by the failure to remove maternal RNAs and to turn off zygotic genome expression.

Conclusions: HFD-induced obesity impaired ovarian morphology and oocyte function in female mice and further led to alterations in the transcriptional landscape of preimplantation embryos at different stages of HFD mice.

单细胞 RNA 测序揭示了高脂饮食对雌性小鼠卵母细胞和早期胚胎发育的影响。
背景:肥胖是一个全球性的健康问题,对包括生殖系统在内的各种人体器官都有不利影响。人类观察数据和一些动物实验数据表明,母体肥胖会损害卵巢功能和早期胚胎发育,但确切的发病机制仍不清楚:方法:我们建立了高脂饮食(HFD)诱导的肥胖雌性小鼠模型,以评估小鼠的全身代谢、卵巢形态和卵母细胞功能。本研究首次采用单细胞 RNA 测序技术来探索高脂饮食诱导肥胖小鼠植入前胚胎不同阶段转录组的变化。结果显示:HFD诱导的肥胖小鼠胚胎植入前不同阶段的转录组图谱发生了改变,并进行了差异基因表达分析、富集分析和蛋白-蛋白相互作用网络分析:结果:HFD诱导的肥胖雌性小鼠表现出糖脂代谢受损和胰岛素抵抗。HFD小鼠卵巢中卵泡总数减少,闭锁卵泡比例增加,颗粒细胞排列不规则。此外,HFD 小鼠卵母细胞体外受精的胚胎发育成熟率显著下降。此外,不同饮食诱导的小鼠植入前胚胎在不同阶段的转录景观也有明显差异。母体到子代的转变也受到了未能去除母体RNA和关闭子代基因组表达的影响:结论:HFD诱导的肥胖损害了雌性小鼠的卵巢形态和卵母细胞功能,并进一步导致了HFD小鼠不同阶段着床前胚胎转录景观的改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Reproductive Biology and Endocrinology
Reproductive Biology and Endocrinology 医学-内分泌学与代谢
CiteScore
7.90
自引率
2.30%
发文量
161
审稿时长
4-8 weeks
期刊介绍: Reproductive Biology and Endocrinology publishes and disseminates high-quality results from excellent research in the reproductive sciences. The journal publishes on topics covering gametogenesis, fertilization, early embryonic development, embryo-uterus interaction, reproductive development, pregnancy, uterine biology, endocrinology of reproduction, control of reproduction, reproductive immunology, neuroendocrinology, and veterinary and human reproductive medicine, including all vertebrate species.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信