The structure and interaction of polymers affects secondary cell wall banding patterns in Arabidopsis.

IF 10 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Plant Cell Pub Date : 2024-10-03 DOI:10.1093/plcell/koae233
Sarah A Pfaff, Edward R Wagner, Daniel J Cosgrove
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引用次数: 0

Abstract

Xylem tracheary elements (TEs) synthesize patterned secondary cell walls (SCWs) to reinforce against the negative pressure of water transport. VASCULAR-RELATED NAC-DOMAIN 7 (VND7) induces differentiation, accompanied by cellulose, xylan, and lignin deposition into banded domains. To investigate the effect of polymer biosynthesis mutations on SCW patterning, we developed a method to induce tracheary element transdifferentiation of isolated protoplasts, by transient transformation with VND7. Our data showed that proper xylan elongation is necessary for distinct cellulose bands, cellulose-xylan interactions are essential for coincident polymer patterns, and cellulose deposition is needed to override the intracellular organization that yields unique xylan patterns. These data indicate that a properly assembled cell wall network acts as a scaffold to direct polymer deposition into distinctly banded domains. We describe the transdifferentiation of protoplasts into TEs, providing an avenue to study patterned SCW biosynthesis in a tissue-free environment and in various mutant backgrounds.

聚合物的结构和相互作用影响拟南芥次生细胞壁的条带模式。
木质部气管元件合成图案化的次生细胞壁(SCW),以抵御水分运输的负压。VASCULAR-RELATED NAC-DOMAIN7 (VND7) 可诱导分化,并伴随纤维素、木聚糖和木质素沉积成带状结构域。为了研究聚合物生物合成突变对 SCW 形态的影响,我们开发了一种方法,通过 VND7 的瞬时转化,诱导离体原生质体的气管元件转分化。我们的数据表明,正确的木聚糖伸长是形成独特纤维素带的必要条件,纤维素-木聚糖相互作用是形成重合聚合物图案的必要条件,而纤维素沉积则是产生独特木聚糖图案的细胞内组织所必需的。这些数据表明,正确组装的细胞壁网络可作为支架,引导聚合物沉积成独特的带状结构域。我们描述了原生质体向气管元件的转分化,为在无组织环境和各种突变背景下研究模式化 SCW 生物合成提供了途径。
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来源期刊
Plant Cell
Plant Cell 生物-生化与分子生物学
CiteScore
16.90
自引率
5.20%
发文量
337
审稿时长
2.4 months
期刊介绍: Title: Plant Cell Publisher: Published monthly by the American Society of Plant Biologists (ASPB) Produced by Sheridan Journal Services, Waterbury, VT History and Impact: Established in 1989 Within three years of publication, ranked first in impact among journals in plant sciences Maintains high standard of excellence Scope: Publishes novel research of special significance in plant biology Focus areas include cellular biology, molecular biology, biochemistry, genetics, development, and evolution Primary criteria: articles provide new insight of broad interest to plant biologists and are suitable for a wide audience Tenets: Publish the most exciting, cutting-edge research in plant cellular and molecular biology Provide rapid turnaround time for reviewing and publishing research papers Ensure highest quality reproduction of data Feature interactive format for commentaries, opinion pieces, and exchange of information in review articles, meeting reports, and insightful overviews.
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