Relationship Between Changes in the Expression Levels of miR-134 and E2F6 in Mediating Control of Apoptosis in NMDA-Induced Glaucomatous Mice.

IF 2.1 4区 医学 Q2 SURGERY
Journal of Investigative Surgery Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI:10.1080/08941939.2024.2389379
Yunli Niu, Houshuo Li, Wenting Han, Ao Rong
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引用次数: 0

Abstract

Objective: This investigation was to determine the relationship between changes in the expression levels of miR-134 and the E2F transcription factor 6 (E2F6) in mediating control of apoptosis in N-methyl-D-aspartate (NMDA)-induced glaucomatous mice.

Methods: Morphological and structural changes were quantitatively analyzed along with apoptosis in the retinal ganglion cell (RGC) layer, internal plexiform layer and RGCs. Glaucomatous RGCs were transfected, and cell viability and apoptosis were examined. The targeting relationship between miR-134 and E2F6 was analyzed, as well as their expression pattern.

Results: Intravitreal injection of NMDA induced a significant reduction in the number of RGCs and thinning of IPL thickness. miR-134 was highly expressed and E2F6 was lowly expressed in glaucoma mice. Suppression of miR-134 or E2F6 overexpression inhibited apoptosis in the glaucomatous RGCs and instead their proliferative activity. MiR-134 targeted inhibition of E2F6 expression. Suppressing rises in E2F6 expression reduced the interfering effect of miR-134 on glaucomatous RGC development.

Conclusion: Depleting miR134 expression increases, in turn, E2F6 expression levels and in turn reduces glaucomatous RGC apoptosis expression.

miR-134 和 E2F6 表达水平的变化与介导 NMDA 诱导的青光眼小鼠凋亡控制之间的关系
研究目的本研究旨在确定在N-甲基-D-天冬氨酸(NMDA)诱导的青光眼小鼠中,miR-134的表达水平变化与E2F转录因子6(E2F6)在介导控制细胞凋亡方面的关系:方法:定量分析视网膜神经节细胞(RGC)层、内丛状层和RGCs的形态和结构变化以及凋亡情况。转染患有青光眼的 RGC,并检测细胞活力和凋亡情况。分析了miR-134和E2F6的靶向关系及其表达模式:结果:玻璃体内注射NMDA会导致RGC数量显著减少,IPL厚度变薄。抑制miR-134或E2F6的过表达可抑制青光眼RGCs的凋亡,并提高其增殖活性。MiR-134 可靶向抑制 E2F6 的表达。抑制 E2F6 表达的升高降低了 miR-134 对青光眼 RGC 发育的干扰作用:结论:消耗 miR134 的表达反过来会增加 E2F6 的表达水平,进而减少青光眼 RGC 的凋亡表达。
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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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