{"title":"Molecular Characterization of a KPC-2- and NDM-1-Producing <i>Klebsiella michiganensis</i> Clinical Isolate in Cerebrospinal Fluid.","authors":"Mingyue Sun, Weiqiang Xiao, Qingxia Xu","doi":"10.2147/IDR.S468895","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong><i>Klebsiella michiganensis</i> is an emerging pathogen. In this context, we characterised a strain fxq isolated from a cerebrospinal fluid specimen of a patient with tentorial meningioma, and the <i>K. michiganensis</i> isolate produced carbapenemases of KPC and NDM types.</p><p><strong>Methods: </strong>The Phoenix 100 Automated Microbiology System, MALDI-TOF and whole-genome sequencing were used to identify the species. Anti-microbial susceptibility testing was also conducted with the Phoenix 100. The plasmid locations of the <i>bla</i> <sub>KPC-2</sub> and <i>bla</i> <sub>NDM-1</sub> genes were determined by S1-nuclease pulsed-field gel electrophoresis and Southern blot. The transfer capacity of plasmids carrying <i>bla</i> <sub>KPC-2</sub> and <i>bla</i> <sub>NDM-1</sub> was investigated by conjugation experiments, and the resistance plasmid stability was evaluated by culture and subculture. <i>K. michiganensis</i> subtypes were identified by multi-locus sequence typing. We performed whole-genome sequencing to confirm species, characterise plasmids and analyse core genes.</p><p><strong>Results: </strong>fxq was originally identified as <i>Klebsiella oxytoca</i> and showed resistance to imipenem and meropenem, but whole-genome sequencing identified it to be <i>K. michiganensis</i>. The strain fxq belonged to the novel sequence type 202 (ST202) and carried the <i>bla</i> <sub>KPC-2</sub> and <i>bla</i> <sub>NDM-1</sub> genes located on the pB_KPC InFIA and pE_NDM IncU plasmids, respectively. The <i>bla</i> <sub>KPC-2</sub>-carrying plasmid was successfully transferred to <i>Escherichia coli</i> EC600 by conjugation, whereas the <i>bla</i> <sub>NDM-1</sub> gene on the pE_NDM plasmid was not. The pB_KPC and pE_NDM plasmids demonstrated high stability.</p><p><strong>Conclusion: </strong>This work is the first report on a carbapenem-resistant clinical isolate <i>K. michiganensis</i> ST202 harbouring the <i>bla</i> <sub>KPC-2</sub> and <i>bla</i> <sub>NDM-1</sub> genes encoded by the IncFIA and IncU plasmids, respectively.</p>","PeriodicalId":13577,"journal":{"name":"Infection and Drug Resistance","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334930/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection and Drug Resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IDR.S468895","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Klebsiella michiganensis is an emerging pathogen. In this context, we characterised a strain fxq isolated from a cerebrospinal fluid specimen of a patient with tentorial meningioma, and the K. michiganensis isolate produced carbapenemases of KPC and NDM types.
Methods: The Phoenix 100 Automated Microbiology System, MALDI-TOF and whole-genome sequencing were used to identify the species. Anti-microbial susceptibility testing was also conducted with the Phoenix 100. The plasmid locations of the blaKPC-2 and blaNDM-1 genes were determined by S1-nuclease pulsed-field gel electrophoresis and Southern blot. The transfer capacity of plasmids carrying blaKPC-2 and blaNDM-1 was investigated by conjugation experiments, and the resistance plasmid stability was evaluated by culture and subculture. K. michiganensis subtypes were identified by multi-locus sequence typing. We performed whole-genome sequencing to confirm species, characterise plasmids and analyse core genes.
Results: fxq was originally identified as Klebsiella oxytoca and showed resistance to imipenem and meropenem, but whole-genome sequencing identified it to be K. michiganensis. The strain fxq belonged to the novel sequence type 202 (ST202) and carried the blaKPC-2 and blaNDM-1 genes located on the pB_KPC InFIA and pE_NDM IncU plasmids, respectively. The blaKPC-2-carrying plasmid was successfully transferred to Escherichia coli EC600 by conjugation, whereas the blaNDM-1 gene on the pE_NDM plasmid was not. The pB_KPC and pE_NDM plasmids demonstrated high stability.
Conclusion: This work is the first report on a carbapenem-resistant clinical isolate K. michiganensis ST202 harbouring the blaKPC-2 and blaNDM-1 genes encoded by the IncFIA and IncU plasmids, respectively.
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ISSN: 1178-6973
Editor-in-Chief: Professor Suresh Antony
An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.