Evaluation of survivin expression and regulating miRNAs of survivin expression in peripheral blood mononuclear cells in systemic lupus erythematous patients.

IF 1.9 4区 医学 Q3 RHEUMATOLOGY
Lupus Pub Date : 2024-10-01 Epub Date: 2024-08-20 DOI:10.1177/09612033241276280
Nasim Bolouri, Reza Mansouri, Elham Farhadi, Samaneh Soltani, Maryam Akhtari, Elham Madreseh, Seyedeh Tahereh Faezi, Saeideh Jafarinejad-Farsangi, Ahmadreza Jamshidi, Mahdi Mahmoudi
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引用次数: 0

Abstract

Background: Systemic lupus erythematosus is a multisystemic rheumatic disease with different clinical features. Disturbance in apoptosis regulation seems to be a major factor in SLE development.

Objective: Survivin plays a key role in mitosis and inhibiting apoptosis. A study was conducted to examine the expression level of survivin and miRNAs that affect survivin transcript levels in patients with SLE.

Methods: We isolated peripheral blood mononuclear cells from 50 inactive SLE patients and 50 healthy controls. RNA is extracted and converted to cDNA. The quantitative real-time polymerase chain reaction is conducted to assess the expression levels of survivin total and its variants with effective miRNAs in PBMCs.

Results: Expression levels of miR-34a-5p (fold change = 1.5, p++ = 0.027), and 218-5p (fold change = 1.5, p++ = 0.020) were significantly increased. While miR-150-5p (fold change = 0.56, p++ = 0.003) was significantly decreased. The mRNA expression of survivin-WT (fold change = 0.63, p++ = 0.002) was significantly downregulated in SLE patients compared to the healthy controls. Survivin total and its two major variants (survivin-2B, and survivin-ΔEx3) did not differ significantly between SLE patients and controls.

Conclusion: Although survivin-TS and its two variants (survivin-2B, and survivin-ΔEx3) were not differently expressed in SLE patients, survivin-WT had altered expression. Despite aberrant miRNA expression in PBMCs from SLE patients, survivin and miRNA expression were not associated with leukopenia. The pathogenesis of SLE disorder might be linked to survivin's other roles in the immune system aside from anti-apoptotic functions.

评估系统性红斑狼疮患者外周血单核细胞中存活素的表达和调控存活素表达的 miRNAs。
背景:系统性红斑狼疮是一种多系统风湿性疾病,具有不同的临床特征。细胞凋亡调节紊乱似乎是系统性红斑狼疮发病的一个主要因素:Survivin 在有丝分裂和抑制细胞凋亡中起着关键作用。本研究旨在检测系统性红斑狼疮患者体内存活素的表达水平以及影响存活素转录水平的 miRNA:我们分离了 50 名非活动性系统性红斑狼疮患者和 50 名健康对照者的外周血单核细胞。提取 RNA 并将其转化为 cDNA。采用实时定量聚合酶链反应评估存活素总表达量及其变体与有效 miRNA 在 PBMCs 中的表达水平:结果:miR-34a-5p(fold change = 1.5,p++ = 0.027)和 218-5p(fold change = 1.5,p++ = 0.020)的表达水平显著增加。而 miR-150-5p(折叠变化 = 0.56,p++ = 0.003)则明显下降。与健康对照组相比,系统性红斑狼疮患者的存活素-WT(折叠变化=0.63,p++=0.002)的mRNA表达明显下调。Survivin总量及其两个主要变体(survivin-2B和survivin-ΔEx3)在系统性红斑狼疮患者和对照组之间没有明显差异:结论:尽管存活素-TS及其两个变体(存活素-2B和存活素-ΔEx3)在系统性红斑狼疮患者中的表达没有差异,但存活素-WT的表达有所改变。尽管系统性红斑狼疮患者的血浆细胞中 miRNA 表达异常,但存活素和 miRNA 的表达与白细胞减少症无关。系统性红斑狼疮的发病机制可能与存活素在免疫系统中除抗凋亡功能外的其他作用有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lupus
Lupus 医学-风湿病学
CiteScore
4.20
自引率
11.50%
发文量
225
审稿时长
1 months
期刊介绍: The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…
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