Utilizing Oral Neutrophil Counts as an Indicator of Oral Inflammation Associated With Periodontal Disease: A Blinded Multicentre Study

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology Pub Date : 2024-08-19 DOI:10.1111/jcpe.14054

Omnia Elebyary, Chunxiang Sun, Elis Angela Batistella, Thomas E. Van Dyke, Samuel B. Low, Sonica Singhal, Howard Tenenbaum, Michael Glogauer

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引用次数: 0

Abstract

Background

Periodontal diseases are chronic inflammatory conditions that require early screening for effective long-term management. Oral neutrophil counts (ONCs) correlate with periodontal inflammation. This study investigates a point-of-care test using a neutrophil enzyme activity (NEA) colorimetric strip for measuring periodontal inflammation.

Methods

This prospective study had two phases. Phase 1 validated the relationship between ONCs and periodontal inflammation with 90 participants. Phase 2 examined the test's applicability in a real-world setting through a multicentre clinical trial with 375 participants at four sites. ONCs were quantified in oral rinses using laboratory-based methods, and the NEA strip was used for ONC stratification. Clinical measures included bleeding on probing (BoP), probing depth (PD) and clinical attachment loss (CAL).

Results

ONCs were significantly elevated in patients with Grade B periodontitis and deep periodontal pockets (PD ≥ 5 mm, CAL ≥ 5 mm). The NEA strip accurately classified patients into high or low ONC categories, showing 80% sensitivity, 82.5% specificity and an AUC of 0.89. It also assessed the effectiveness of periodontal therapy in reducing ONC and inflammation. The test was user-friendly, with no reported discomfort among patients.

Conclusion

The NEA strip is a user-friendly and rapid screening tool for detecting high ONCs associated with periodontal inflammation and for evaluating the effectiveness of periodontal therapy.

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利用口腔中性粒细胞计数作为牙周病相关口腔炎症的指标：盲法多中心研究

背景：牙周疾病是一种慢性炎症，需要及早筛查，以便进行有效的长期治疗。口腔中性粒细胞计数（ONCs）与牙周炎症相关。本研究调查了一种使用中性粒细胞酶活性（NEA）比色条测量牙周炎症的护理点检测方法：这项前瞻性研究分为两个阶段。方法：这项前瞻性研究分为两个阶段，第一阶段在 90 名参与者中验证了中性粒细胞酶与牙周炎症之间的关系。第二阶段通过在四个地点对 375 名参与者进行多中心临床试验，检验该测试在实际环境中的适用性。采用实验室方法对口腔冲洗液中的ONC进行量化，并使用NEA条对ONC进行分层。临床测量包括探诊出血（BoP）、探诊深度（PD）和临床附着丧失（CAL）：结果：B级牙周炎和深牙周袋（PD≥5 mm，CAL≥5 mm）患者的ONC明显升高。NEA条带能准确地将患者分为高或低ONC类别，灵敏度为80%，特异性为82.5%，AUC为0.89。它还能评估牙周治疗在减少 ONC 和炎症方面的效果。该测试操作简便，患者无不适反应：NEA条是一种方便用户使用的快速筛查工具，可用于检测与牙周炎症相关的高ONC，并评估牙周治疗的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Periodontology 医学-牙科与口腔外科

CiteScore

13.30

自引率

10.40%

发文量

175

审稿时长

3-8 weeks

期刊介绍： Journal of Clinical Periodontology was founded by the British, Dutch, French, German, Scandinavian, and Swiss Societies of Periodontology. The aim of the Journal of Clinical Periodontology is to provide the platform for exchange of scientific and clinical progress in the field of Periodontology and allied disciplines, and to do so at the highest possible level. The Journal also aims to facilitate the application of new scientific knowledge to the daily practice of the concerned disciplines and addresses both practicing clinicians and academics. The Journal is the official publication of the European Federation of Periodontology but wishes to retain its international scope. The Journal publishes original contributions of high scientific merit in the fields of periodontology and implant dentistry. Its scope encompasses the physiology and pathology of the periodontium, the tissue integration of dental implants, the biology and the modulation of periodontal and alveolar bone healing and regeneration, diagnosis, epidemiology, prevention and therapy of periodontal disease, the clinical aspects of tooth replacement with dental implants, and the comprehensive rehabilitation of the periodontal patient. Review articles by experts on new developments in basic and applied periodontal science and associated dental disciplines, advances in periodontal or implant techniques and procedures, and case reports which illustrate important new information are also welcome.