Biotechnological application of Aureobasidium spp. as a promising chassis for biosynthesis of ornithine-urea cycle-derived bioproducts.

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Khin Myo Myo Tint, Xin Wei, Peng Wang, Guang-Lei Liu, Mei Zhang, Zhen-Ming Chi, Zhe Chi
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引用次数: 0

Abstract

The ornithine-urea cycle (OUC) in fungal cells has biotechnological importance and many physiological functions and is closely related to the acetyl glutamate cycle (AGC). Fumarate can be released from argininosuccinate under the catalysis of argininosuccinate lyase in OUC which is regulated by the Ca2+ signaling pathway and over 93.9 ± 0.8 g/L fumarate can be yielded by the engineered strain of Aureobasidium pullulans var. aubasidani in the presence of CaCO3. Furthermore, 2.1 ± 0.02 mg of L-ornithine (L-Orn)/mg of the protein also can be synthesized via OUC by the engineered strains of Aureobasidum melanogenum. Fumarate can be transformed into many drugs and amino acids and L-Orn can be converted into siderophores (1.7 g/L), putrescine (33.4 g/L) and L-piperazic acid (L-Piz) (3.0 g/L), by different recombinant strains of A. melanogenum. All the fumarate, L-Orn, siderophore, putrescine and L-Piz have many applications. As the yeast-like fungi and the promising chassis, Aureobasidium spp, have many advantages over any other fungal strains. Further genetic manipulation and bioengineering will enhance the biosynthesis of fumarate and L-Orn and their derivates.

Aureobasidium spp.作为鸟氨酸-尿素循环衍生生物产品生物合成底盘的生物技术应用。
真菌细胞中的鸟氨酸脲循环(OUC)具有重要的生物技术意义和多种生理功能,与乙酰谷氨酸循环(AGC)密切相关。在 OUC 中,在精氨酸琥珀酸裂解酶的催化下,富马酸可从精氨酸琥珀酸中释放出来,而精氨酸琥珀酸裂解酶受 Ca2+ 信号通路的调控,在 CaCO3 的存在下,工程菌株 Aureobasidium pullulans var.此外,Aureobasidum melanogenum 的工程菌株也能通过 OUC 合成 2.1 ± 0.02 mg L-鸟氨酸(L-Orn)/mg 蛋白质。富马酸盐可转化为多种药物和氨基酸,L-Orn可通过不同的重组菌株转化为苷酸(1.7 g/L)、腐胺(33.4 g/L)和L-哌嗪酸(L-Piz)(3.0 g/L)。所有富马酸盐、L-Orn、苷元、腐胺和 L-Piz 都有很多用途。与其他真菌菌株相比,类酵母真菌和有前途的底盘 Aureobasidium spp 具有许多优势。进一步的遗传操作和生物工程将提高富马酸和 L-Orn 及其衍生物的生物合成能力。
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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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