Apoptosis induction and inhibition of invasion and migration in gastric cancer cells by Isoorientin studied using network pharmacology.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-08-19 DOI:10.1186/s12906-024-04605-z

Dan Song, Maosheng Chen, Xiangjun Chen, Jiaojiao Xu, Siqi Wu, Yaxin Lyu, Qin Zhao

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引用次数: 0

Abstract

Background: To investigate the effects of Isoorientin on the apoptosis, proliferation, invasion, and migration of human gastric cancer cells (HGC27 cells).

Methods: We used network pharmacology to predict the targets of drugs and diseases. The CCK-8 assay was used to determine the effects of Isoorientin on the proliferation of HGC27 cells. Flow cytometry was employed to analyze the effects of Isoorientin on cell apoptosis and cell cycle distribution of HGC27 cells. Scratch test and transwell chamber test were conducted to assess the effects of Isoorientin on invasion and migration, respectively. Additionally, qPCR and western blot were performed to examine the impact of Isoorientin on apoptosis-related genes and protein expression, respectively.

Results: The Isoorientin significantly inhibited the proliferation, migration, and invasion of HGC27 cells compared to the control group. Furthermore, Isoorientin induced apoptosis in HGC27 cells by upregulating the relative expression of Bax and caspase-3 while downregulating the relative expression of p-PI3K, p-AKT, and Bcl-2 proteins.

Conclusion: The Isoorientin exhibits inhibitory effects on the proliferation, invasion, and migration of HGC27 cells, and induces apoptosis in gastric cancer cells.

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利用网络药理学研究异连翘素诱导胃癌细胞凋亡并抑制其侵袭和迁移的作用。

背景：研究异连翘素对人胃癌细胞（HGC27）凋亡、增殖、侵袭和迁移的影响：研究异连翘素对人胃癌细胞（HGC27 细胞）凋亡、增殖、侵袭和迁移的影响：方法：我们利用网络药理学预测药物和疾病的靶点。方法：我们利用网络药理学预测药物和疾病的靶点，采用 CCK-8 检测法确定异连翘素对 HGC27 细胞增殖的影响。流式细胞术分析了异连蛋白对HGC27细胞凋亡和细胞周期分布的影响。划痕试验和经孔室试验分别评估了异连蛋白对侵袭和迁移的影响。此外，还分别进行了qPCR和Western blot检测异连蛋白对细胞凋亡相关基因和蛋白表达的影响：结果：与对照组相比，异连蛋白能明显抑制HGC27细胞的增殖、迁移和侵袭。此外，异连蛋白通过上调Bax和caspase-3的相对表达，同时下调p-PI3K、p-AKT和Bcl-2蛋白的相对表达，诱导HGC27细胞凋亡：结论：异连翘素对HGC27细胞的增殖、侵袭和迁移具有抑制作用，并能诱导胃癌细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567