Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari
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引用次数: 0

Abstract

Kojic acid derivatives are useful in the cosmetics and pharmaceutical industries. The current investigation focuses on the search for a safe and environmentally friendly newer whole-cell biocatalyst for the synthesis of kojic acid derivative especially 2-amino-6-(hydroxymethyl)-8-oxo-4-phenyl-4,8-dihydropyrano[3,2-b]pyran-3-carbonitrile (APhCN). In this context, a total of six cultures were isolated from fecal samples of infants and subjected to probiotic characterization followed by screening as whole cell biocatalyst (WCB). In this multicomponent reaction, benzaldehyde, malononitrile, and kojic acid were used to synthesize APhCN at room temperature under aqueous conditions. The screening of potent whole cell biocatalyst (WCB) from isolated cultures was done by comparing reaction time and percent yield. The potent WCB gave a good yield of 95% within 15 h of time and hence further characterized biochemically and identified as Lentilactobacillus farraginis by using 16S rRNA gene sequencing. Lactobacilli having GRAS (generally regarded as safe) status and being able to carry out this transformation under moderate reaction conditions with easy recovery of both product and biocatalyst, it has the potential to replace some of the chemical catalytic methods.

Abstract Image

远拉氏扁豆乳杆菌 FSI (3):水生条件下合成曲酸衍生物的全细胞生物催化剂。
曲酸衍生物可用于化妆品和制药业。目前的研究重点是寻找一种安全、环保的新型全细胞生物催化剂,用于合成曲酸衍生物,特别是 2-氨基-6-(羟甲基)-8-氧代-4-苯基-4,8-二氢吡喃并[3,2-b]吡喃-3-甲腈(APhCN)。在这种情况下,我们从婴儿粪便样本中分离出了六种培养物,并对其进行了益生菌鉴定,然后筛选出了全细胞生物催化剂(WCB)。在这个多组分反应中,使用苯甲醛、丙二腈和曲酸在室温水溶液条件下合成 APhCN。通过比较反应时间和收率百分比,从分离培养物中筛选出了强效全细胞生物催化剂(WCB)。这种强效全细胞生物催化剂在 15 小时内的产率高达 95%,因此通过 16S rRNA 基因测序对其进行了进一步的生化鉴定,并确定其为远拉氏扁豆乳杆菌(Lentilactobacillus farraginis)。乳酸杆菌具有 GRAS(一般认为是安全的)地位,能够在温和的反应条件下进行这种转化,而且产品和生物催化剂都易于回收,因此有可能取代某些化学催化方法。
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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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