Genome-wide identification of HSP90 gene family in Rosa chinensis and its response to salt and drought stresses.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-09-01 Epub Date: 2024-08-18 DOI:10.1007/s13205-024-04052-0
Jun Xu, Shuangwei Liu, Yueming Ren, Yang You, Zhifang Wang, Yongqiang Zhang, Xinjie Zhu, Ping Hu
{"title":"Genome-wide identification of <i>HSP90</i> gene family in <i>Rosa chinensis</i> and its response to salt and drought stresses.","authors":"Jun Xu, Shuangwei Liu, Yueming Ren, Yang You, Zhifang Wang, Yongqiang Zhang, Xinjie Zhu, Ping Hu","doi":"10.1007/s13205-024-04052-0","DOIUrl":null,"url":null,"abstract":"<p><p>Heat shock protein 90 (HSP90) is important for many organisms, including plants. Based on the whole genome information, the gene number, gene structure, evolutionary relationship, protein structure, and active site of the HSP90 gene family in <i>Rosa chinensis</i> and <i>Rubus idaeus</i> were determined, and the expression of the <i>HSP90</i> gene under salt, and drought stresses in two rose varieties Wangxifeng and Sweet Avalanche were analyzed. Six and eight <i>HSP90</i> genes were identified from <i>R. chinensis</i> and <i>Ru. idaeus</i>, respectively. Phylogenetic analysis revealed that the analyzed genes were divided into two Groups and four subgroups (Classes 1a, 1b, 2a, and 2b). Although members within the same classes displayed highly similar gene structures, while the gene structures and conserved domains of Group 1 (Class 1a and 1b) and the Group 2 (Class 2a and 2b) are different. Tandem and segmental duplication genes were found in <i>Ru. idaeus</i>, but not in <i>R. chinensis</i>, perhaps explaining the difference in <i>HSP90</i> gene quantity in the two analyzed species. Analysis of cis-acting elements revealed abundant abiotic stress, photolight-response, and hormone-response elements in <i>R. chinensis</i> HSP90s. qRT-PCR analysis suggested that <i>RcHSP90-1-1</i>, <i>RcHSP90-5-1</i> and <i>RcHSP90-6-1</i> in Sweet Avalanche and Wangxifeng varieties played important regulatory roles under salt and drought stress. The analysis of protein structure and active sites indicate that the potential different roles of <i>RcHSP90-1-1</i>, <i>RcHSP90-5-1</i>, and <i>RcHSP90-6-1</i> in salt and drought stresses may come from the differences of corresponding protein structures and activation sites. These data will provide information for the breeding of rose varieties with high stress resistance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04052-0.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330952/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"3 Biotech","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13205-024-04052-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/18 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Heat shock protein 90 (HSP90) is important for many organisms, including plants. Based on the whole genome information, the gene number, gene structure, evolutionary relationship, protein structure, and active site of the HSP90 gene family in Rosa chinensis and Rubus idaeus were determined, and the expression of the HSP90 gene under salt, and drought stresses in two rose varieties Wangxifeng and Sweet Avalanche were analyzed. Six and eight HSP90 genes were identified from R. chinensis and Ru. idaeus, respectively. Phylogenetic analysis revealed that the analyzed genes were divided into two Groups and four subgroups (Classes 1a, 1b, 2a, and 2b). Although members within the same classes displayed highly similar gene structures, while the gene structures and conserved domains of Group 1 (Class 1a and 1b) and the Group 2 (Class 2a and 2b) are different. Tandem and segmental duplication genes were found in Ru. idaeus, but not in R. chinensis, perhaps explaining the difference in HSP90 gene quantity in the two analyzed species. Analysis of cis-acting elements revealed abundant abiotic stress, photolight-response, and hormone-response elements in R. chinensis HSP90s. qRT-PCR analysis suggested that RcHSP90-1-1, RcHSP90-5-1 and RcHSP90-6-1 in Sweet Avalanche and Wangxifeng varieties played important regulatory roles under salt and drought stress. The analysis of protein structure and active sites indicate that the potential different roles of RcHSP90-1-1, RcHSP90-5-1, and RcHSP90-6-1 in salt and drought stresses may come from the differences of corresponding protein structures and activation sites. These data will provide information for the breeding of rose varieties with high stress resistance.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04052-0.

Abstract Image

蔷薇属植物 HSP90 基因家族的全基因组鉴定及其对盐和干旱胁迫的响应
热休克蛋白90(HSP90)对包括植物在内的许多生物都很重要。基于全基因组信息,确定了蔷薇和茜草中HSP90基因家族的基因数目、基因结构、进化关系、蛋白结构和活性位点,并分析了盐胁迫和干旱胁迫下HSP90基因在两个蔷薇品种旺喜丰和甜崩中的表达情况。结果表明,盐胁迫和干旱胁迫下的HSP90基因表达量分别为6个和8个。系统进化分析表明,所分析的基因分为两个组和四个亚组(1a、1b、2a 和 2b)。虽然同类成员的基因结构高度相似,但第 1 组(1a 类和 1b 类)和第 2 组(2a 类和 2b 类)的基因结构和保守结构域不同。在Ru. idaeus中发现了串联和节段重复基因,而在R. chinensis中却没有发现,这或许可以解释两个分析物种中HSP90基因数量的差异。qRT-PCR分析表明,甜崩和旺西丰品种中的RcHSP90-1-1、RcHSP90-5-1和RcHSP90-6-1在盐胁迫和干旱胁迫下发挥了重要的调控作用。对蛋白质结构和活性位点的分析表明,RcHSP90-1-1、RcHSP90-5-1和RcHSP90-6-1在盐胁迫和干旱胁迫中的潜在不同作用可能来自于相应蛋白质结构和激活位点的差异。这些数据将为培育具有高抗逆性的玫瑰品种提供信息:在线版本包含补充材料,可查阅 10.1007/s13205-024-04052-0。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信