DNA Barcoding of Catfishes (Order: Siluriformes) with New Records of Two Species from Eastern Nepal

IF 0.7 4区 农林科学 Q4 FISHERIES
Jash Hang Limbu, Dipak Rajbanshi, Laxman Khanal, Wang Hui, Ram Chandra Adhikari, Sunita Thapa, Jin Quan Yang, Chenhong Li
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引用次数: 0

Abstract

The freshwater bodies of Nepal host a great deal of fish diversity; however, the molecular study of Nepalese fish is still in its early stages. Conservation management tactics require knowledge of the real species composition, often impeded by descriptions of intermittent species based on traditional taxonomy. Catfish, one of the most overfished taxa on the planet, are abundant and heavily exploited in the eastern region of Nepal. Hence, actual catfish diversity in this region needs to be examined using a combined morphological and molecular DNA barcoding approach. In this study, we sequenced the cytochrome oxidase subunit I (COI) gene in Nepalese fish species for the first time and used it for molecular identification. Altogether, 99 specimens representing 29 species belonging to 21 genera and 8 families were successfully barcoded. They were found to be 98.5–100% identical to the known sequences deposited in the GenBank and BOLD databases. Of these, two species, Amblyceps arunachalense and Erethistoides sicula, were recorded for the first time in Nepal. Out of 29 species, one species was found to be Endangered, one species under Vulnerable, two species under Near Threatened, one species under Data Deficient, and 24 species under Least Concern. The intraspecific genetic distances within species ranged from 0 to 5%. The highest intraspecific genetic distances (Kimura 2-parameter) were found among the individuals of two species, namely, E. sicula (4%) and Glyptothorax telchitta (5%), indicating the presence of putative (sub)species. The interspecific genetic distances among the catfishes in eastern Nepal ranged between 8.47% (between Glyptothorax trilineatus and G. cavia) and 26.38% (between Amblyceps arunachalense and Chaca chaca). The GC content at the 1st, 2nd, and 3rd positions of the codons was found to be 42.4%, 49.3%, and 43.8%, respectively. These findings will support future initiatives for fish resource inventory, monitoring, and management. It is also an important input to the DNA barcode library of catfish in the eastern region of Nepal.

Abstract Image

鲶鱼(目:丝形目)的 DNA 条形码以及尼泊尔东部两个鱼种的新记录
尼泊尔淡水水体中的鱼类种类繁多,但对尼泊尔鱼类的分子研究仍处于早期阶段。保护管理策略需要了解真实的物种组成,而基于传统分类法的间歇性物种描述往往会阻碍这种了解。鲶鱼是地球上被过度捕捞最严重的类群之一,在尼泊尔东部地区资源丰富,开发严重。因此,需要使用形态学和分子 DNA 条形码相结合的方法来研究该地区鲶鱼的实际多样性。在这项研究中,我们首次对尼泊尔鱼类物种的细胞色素氧化酶亚单位 I(COI)基因进行了测序,并将其用于分子鉴定。我们成功地对隶属于 21 属 8 科 29 种鱼类的 99 个标本进行了条形码编码。结果发现,它们与保存在 GenBank 和 BOLD 数据库中的已知序列有 98.5-100% 的相同度。其中,两个物种(Amblyceps arunachalense 和 Erethistoides sicula)是首次在尼泊尔记录到。在 29 个物种中,有 1 个物种被认定为濒危物种,1 个物种被认定为易危物种,2 个物种被认定为近危物种,1 个物种被认定为数据不足物种,24 个物种被认定为最不关注物种。物种间的种内遗传距离从 0%到 5%不等。两个物种个体之间的种内遗传距离(木村 2 参数)最大,分别为 E. sicula(4%)和 Glyptothorax telchitta(5%),表明存在假定(亚)物种。尼泊尔东部鲶鱼种间遗传距离介于 8.47%(Glyptothorax trilineatus 和 G. cavia 之间)和 26.38%(Amblyceps arunachalense 和 Chaca chaca 之间)之间。密码子第 1、第 2 和第 3 位的 GC 含量分别为 42.4%、49.3% 和 43.8%。这些发现将为今后的鱼类资源清查、监测和管理提供支持。这也是对尼泊尔东部地区鲶鱼 DNA 条形码库的重要投入。
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来源期刊
Journal of Applied Ichthyology
Journal of Applied Ichthyology 生物-海洋与淡水生物学
CiteScore
2.30
自引率
11.10%
发文量
73
审稿时长
3-8 weeks
期刊介绍: The Journal of Applied Ichthyology publishes articles of international repute on ichthyology, aquaculture, and marine fisheries; ichthyopathology and ichthyoimmunology; environmental toxicology using fishes as test organisms; basic research on fishery management; and aspects of integrated coastal zone management in relation to fisheries and aquaculture. Emphasis is placed on the application of scientific research findings, while special consideration is given to ichthyological problems occurring in developing countries. Article formats include original articles, review articles, short communications and technical reports.
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