Multi-omics integrative analysis provided new insights into alkaline stress in alfalfa

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-08-18 DOI:10.1016/j.plaphy.2024.109048

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Abstract

Saline-alkali stress is one of the main abiotic stresses that limits plant growth. Salt stress has been widely studied, but alkaline salt degradation caused by NaHCO₃ has rarely been investigated. In the present study, the alfalfa cultivar ‘Zhongmu No. 1′ was treated with 50 mM NaHCO₃ (0, 4, 8, 12 and 24 h) to study the resulting enzyme activity and changes in mRNA, miRNA and metabolites in the roots. The results showed that the enzyme activity changed significantly after alkali stress treatment. The genomic analysis revealed 14,970 differentially expressed mRNAs (DEMs), 53 differentially expressed miRNAs (DEMis), and 463 differentially accumulated metabolites (DAMs). Combined analysis of DEMs and DEMis revealed that 21 DEMis negatively regulated 42 DEMs. In addition, when combined with Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DEMs and DAMs, we found that phenylpropanoid biosynthesis, flavonoid biosynthesis, starch and sucrose metabolism and plant hormone signal transduction played important roles in the alkali stress response. The results of this study further elucidated the regulatory mechanism underlying the plant response to alkali stress and provided valuable information for the breeding of new saline-alkaline tolerance plant varieties.

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多组学综合分析为了解紫花苜蓿的碱性胁迫提供了新思路

盐碱胁迫是限制植物生长的主要非生物胁迫之一。盐胁迫已被广泛研究，但由 NaHCO3 引起的碱性盐降解却鲜有研究。本研究以紫花苜蓿栽培品种 "中牧 1 号 "为研究对象，用 50 mM NaHCO3（0、4、8、12 和 24 h）处理紫花苜蓿，研究其产生的酶活性以及根中 mRNA、miRNA 和代谢产物的变化。结果表明，碱胁迫处理后酶活性发生了显著变化。基因组分析发现了 14,970 个差异表达的 mRNA（DEMs）、53 个差异表达的 miRNA（DEMis）和 463 个差异积累的代谢物（DAMs）。对 DEMs 和 DEMis 的综合分析表明，21 个 DEMis 负向调节 42 个 DEMs。此外，结合京都基因和基因组百科全书（KEGG）对 DEMs 和 DAMs 的分析，我们发现苯丙类生物合成、黄酮类生物合成、淀粉和蔗糖代谢以及植物激素信号转导在碱胁迫响应中发挥了重要作用。研究结果进一步阐明了植物对碱胁迫响应的调控机制，为培育耐盐碱植物新品种提供了宝贵的信息。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.

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