The genus Coelastrella (Chlorophyceae, Chlorophyta): molecular species delimitation, biotechnological potential, and description of a new species Coelastrella affinis sp. nov., based on an integrative taxonomic approach

IF 1.8 3区生物学 Q4 MICROBIOLOGY

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-08-19 DOI:10.1007/s10482-024-02008-1

E. Krivina, M. Sinetova, E. Zadneprovskaya, M. Ivanova, A. Starikov, K. Shibzukhova, E. Lobakova, Yu. Bukin, A. Portnov, A. Temraleeva

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Abstract

Despite the long research history on the genus Coelastrella, its species diversity and biotechnological potential have not been fully explored. For the first time, cluster analysis of morphological characteristics was done in the representatives of the said genus. The results obtained have shown that morphological similarity does not necessarily indicate a molecular genetic relationship. It the light of it, the taxonomic status of species can reliably be determined using specific DNA region, such as 18S–ITS1–5.8S–ITS2. The V4 and V9 regions of gene 18S rRNA are relatively conservative fragments which are not suitable for species identification. The ITS2 can be used as a “short barcode”. Among the advanced machine methods for delimitation species, the most effective algorithm for distinguishing Coelastrella species was the Generalized Mixed Yule Coalescent (GMYC) method. This paper represented for the first time our comprehensive review of the works devoted to the analysis of the biotechnological potential of representatives of the genus Coelastrella and shows that fatty acid composition of the three main chemogroups within the studied genus differs. In the future, this may form the basis for predicting the composition of the fatty acid profile of new strains, which is important while searching for organisms with specified biotechnological properties. In conclusion, an integrative approach was employed to describe Coelastrella affinis sp. nov., a new species of the genus Coelastrella with high biotechnological potential. Also, a new description of C. thermophila var. astaxanthina comb. nov. was proposed.

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Coelastrella属（叶绿藻纲，叶绿目）：分子物种划分、生物技术潜力以及基于综合分类学方法的新物种Coelastrella affinis sp.

尽管对鹅掌楸属（Coelastrella）的研究历史悠久，但其物种多样性和生物技术潜力尚未得到充分发掘。我们首次对该属的代表物种进行了形态特征聚类分析。结果表明，形态上的相似性并不一定表示分子遗传关系。有鉴于此，利用特定的 DNA 区域（如 18S-ITS1-5.8S-ITS2 区域）可以可靠地确定物种的分类地位。18S rRNA 基因的 V4 和 V9 区域是相对保守的片段，不适合用于物种鉴定。ITS2 可用作 "短条形码"。在先进的机器物种划分方法中，区分 Coelastrella 物种最有效的算法是广义混合余乐凝聚法（GMYC）。本文是我们首次对专门分析鹅掌楸属代表生物技术潜力的著作进行的全面回顾，并表明所研究的鹅掌楸属中三个主要化学组的脂肪酸组成是不同的。未来，这可能成为预测新菌株脂肪酸组成的基础，这对于寻找具有特定生物技术特性的生物体非常重要。总之，本研究采用综合方法描述了 Coelastrella affinis sp.此外，还对 C. thermophila var.

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来源期刊

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 生物-微生物学

CiteScore

5.60

自引率

11.50%

发文量

104

审稿时长

3 months

期刊介绍： Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.