A prospective study evaluating the adhesive tape impression for the diagnosis of dermatophytosis in dogs and cats.

IF 1.9 3区农林科学 Q3 DERMATOLOGY

Veterinary dermatology Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI:10.1111/vde.13284

Courtney B Ludwig, Sophie A Tyler, Tatiana Lima, Linda J Vogelnest

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Abstract

Background: Potential contagion and zoonotic risk make rapid diagnosis of dermatophytosis important amongst companion animals.

Hypothesis/objectives: To compare the adhesive tape impression (ATI), trichogram, Wood's lamp (WL) examination and fungal culture for diagnosis of dermatophytosis, and the ATI and trichogram for ease of use.

Animals: Five dogs and 15 cats with consistent lesions, history and at least two positive dermatophyte test results.

Materials and methods: After WL examination, one representative lesion per patient was sampled for ATI, trichogram and fungal culture. Microscopic detection of fungal elements on ATI and trichogram were objectively and subjectively compared. The ability of all tests to correctly detect dermatophytosis, and the ability of ATI and trichogram to detect fungal elements in >20 high-powered fields (HPF) were compared using Fisher's exact tests.

Results: ATI, trichogram, fungal culture and WL were positive in 100% (20 of 20), 90% (18 of 20), 95% (19 of 20) and 65% (13 of 20) of patients, respectively, with a significant difference between WL and ATI (p = 0.0499). Fungal culture revealed Microsporum. canis (n = 16), Nannizzia gypsea (n = 1), Trichophyton mentagrophytes (n = 1) and T. rubrum (n = 1). Fungal elements were detected in >20 HPF more frequently with ATI (18 of 20) than trichogram (11 of 20) (p = 0.017), and detection required careful scanning to distinguish from background debris more often with trichogram. Fungal elements were located with neutrophil clusters and keratinocyte rafts in addition to abnormal hair fragments more frequently on ATI.

Conclusions and clinical relevance: ATI had the highest ability to correctly diagnose dermatophytosis, and increased ease of detecting fungal elements compared to trichogram.

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一项前瞻性研究，评估用于诊断猫狗皮癣菌病的胶带印模。

背景：潜在的传染性和人畜共患病风险使皮癣病的快速诊断在伴侣动物中变得非常重要：比较胶布压片法（ATI）、滴虫造影法、伍德灯（WL）检查法和真菌培养法诊断皮癣病的效果，并比较胶布压片法和滴虫造影法的易用性：动物：5 只狗和 15 只猫，病变、病史一致，至少有两次皮癣菌检测结果呈阳性：在 WL 检查后，对每位患者的一个代表性病灶进行取样，以进行 ATI、滴虫和真菌培养。客观和主观地比较了 ATI 和滴虫造影对真菌成分的显微检测。使用费雪精确检验比较了所有检测方法正确检测皮癣的能力，以及 ATI 和滴虫造影在大于 20 个高倍视野（HPF）中检测真菌成分的能力：结果：ATI、trichogram、真菌培养和 WL 分别在 100%（20 例中有 20 例）、90%（20 例中有 18 例）、95%（20 例中有 19 例）和 65%（20 例中有 13 例）的患者中呈阳性，WL 和 ATI 之间差异显著（p = 0.0499）。真菌培养显示有犬小孢子菌（16 个）、Nannizzia gypsea（1 个）、门冬癣毛癣菌（1 个）和红癣毛癣菌（1 个）。使用 ATI（20 例中的 18 例）比使用 Trichogram（20 例中的 11 例）更经常在 >20 HPF 中检测到真菌成分（p = 0.017），使用 Trichogram 更经常需要仔细扫描才能将真菌成分与背景碎片区分开来。在 ATI 上，除了异常毛发碎片外，真菌成分与中性粒细胞集群和角质细胞筏的位置也更接近：ATI正确诊断皮癣的能力最高，与毛囊造影相比更容易检测到真菌成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary dermatology 农林科学-兽医学

CiteScore

3.20

自引率

21.40%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities