Genome-wide analysis of Citrus medica ABC transporters reveals the regulation of fruit development by CmABCB19 and CmABCC10

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-08-10 DOI:10.1016/j.plaphy.2024.109027

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引用次数: 0

Abstract

ATP-binding cassette (ABC) transporters are vital for plant growth and development as they facilitate the transport of essential molecules. Despite the family's significance, limited information exists about its functional distinctions in Citrus medica. Our study identified 119 genes encoding ABC transporter proteins in the C. medica genome. Through an evolutionary tree and qPCR analysis, two ABC genes, CmABCB19 and CmABCC10, were implicated in C. medica fruit development, showing upregulation in normal fruits compared to malformed fruits. CmABCB19 was found to localize to the plasma membrane of Nicotiana tabacum, exhibiting indole-3-acetic acid (IAA) efflux activity in the yeast mutant strain yap1. CmABCC10, a tonoplast-localized transporter, exhibited efflux of diosmin, nobiletin, and naringin, with rutin influx in strain ycf1. Transgenic expression of CmABCB19 and CmABCC10 in Arabidopsis thaliana induced alterations in auxin and flavonoid content, impacting silique and seed size. This effect was attributed to the modulation of structural genes in the auxin biosynthesis (YUC5/9, CYP79B2, CYP83B1, SUR1) and flavonoid biosynthesis (4CL2/3, CHS, CHI, FLS1/3) pathways. In summary, the functional characterization of CmABCB19 and CmABCC10 illuminates auxin and flavonoid transport, offering insights into their interplay with biosynthetic pathways and providing a foundation for understanding the transporter's role in fruit development.

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柑橘 ABC 转运体的全基因组分析揭示了 CmABCB19 和 CmABCC10 对果实发育的调控作用

ATP 结合盒（ABC）转运体对植物的生长和发育至关重要，因为它们能促进重要分子的转运。尽管该家族意义重大，但有关其在柑橘中功能差异的信息却很有限。我们的研究在柑橘基因组中发现了 119 个编码 ABC 转运体蛋白的基因。通过进化树和 qPCR 分析，发现 CmABCB19 和 CmABCC10 这两个 ABC 基因与枸橘果实的发育有关，与畸形果实相比，它们在正常果实中表现出上调。研究发现，CmABCB19 定位于烟草的质膜，在酵母突变株 yap1 中表现出吲哚-3-乙酸（IAA）外流活性。在拟南芥中转基因表达 CmABCB19 和 CmABCC10 会诱导辅酶和类黄酮含量的改变，从而影响子房和种子的大小。这种影响归因于对辅助素生物合成（YUC5/9、CYP79B2、CYP83B1、SUR1）和黄酮类化合物生物合成（4CL2/3、CHS、CHI、FLS1/3）途径中结构基因的调节。总之，CmABCB19 和 CmABCC10 的功能表征揭示了叶绿素和黄酮类化合物的转运，提供了它们与生物合成途径相互作用的见解，为了解转运体在果实发育中的作用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.