Crescent-shaped Janus nanoassemblies of gold nanoparticles and AIEgens: Enhancing plasmonic and fluorescent activities for colorimetric and ratiometric fluorescence lateral flow immunoassay
{"title":"Crescent-shaped Janus nanoassemblies of gold nanoparticles and AIEgens: Enhancing plasmonic and fluorescent activities for colorimetric and ratiometric fluorescence lateral flow immunoassay","authors":"","doi":"10.1016/j.nantod.2024.102452","DOIUrl":null,"url":null,"abstract":"<div><p>Herein, crescent-shaped Janus nanoassemblies (Au-AIENPs) with high plasmonic and fluorescent activities are prepared by co-assembling oleylamine-coated gold nanoparticles (OA-AuNPs) and red-emitting aggregation-induced emission luminogens (AIEgens) in separate compartments of polymer nanoparticles. The obtained Au-AIENPs show typical Janus heterostructures, where AIEgens preferentially aggregate to form fluorescent core and OA-AuNPs are distributed in the polymer matrix to form a crescent-shaped plasmonic shell, thus resulting in effective spatial separation of OA-AuNPs and AIEgens to achieve the balance between plasmonic and fluorescent signals and reduce the mutual interference between signals. Taking advantage of the excellent plasmonic and fluorescent activities of Au-AIENPs, we successfully established a colorimetric and ratiometric fluorescence dual-mode lateral flow immunoassay (Au-AIENPs-RLFIA) for the visual and quantitative detection of aflatoxin B1 (AFB1) in corn sample. Under the developed conditions, the visual detection limit (vLOD) of the Au-AIENPs-RLFIA for colorimetric and ratiomentic fluorescence detection was 0.62 ng/mL and 0.02 ng/mL, respectively, while the quantitative LOD (qLOD) for ratiomentic fluorescence mode was as low as 0.0076 ng/mL. The above results indicate that the designed Janus Au-AIENPs are promising as dual-signal output probes and hold great potential for improving flexible dual-mode detection of various targets on the LFIA platform.</p></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":null,"pages":null},"PeriodicalIF":13.2000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Today","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1748013224003086","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Herein, crescent-shaped Janus nanoassemblies (Au-AIENPs) with high plasmonic and fluorescent activities are prepared by co-assembling oleylamine-coated gold nanoparticles (OA-AuNPs) and red-emitting aggregation-induced emission luminogens (AIEgens) in separate compartments of polymer nanoparticles. The obtained Au-AIENPs show typical Janus heterostructures, where AIEgens preferentially aggregate to form fluorescent core and OA-AuNPs are distributed in the polymer matrix to form a crescent-shaped plasmonic shell, thus resulting in effective spatial separation of OA-AuNPs and AIEgens to achieve the balance between plasmonic and fluorescent signals and reduce the mutual interference between signals. Taking advantage of the excellent plasmonic and fluorescent activities of Au-AIENPs, we successfully established a colorimetric and ratiometric fluorescence dual-mode lateral flow immunoassay (Au-AIENPs-RLFIA) for the visual and quantitative detection of aflatoxin B1 (AFB1) in corn sample. Under the developed conditions, the visual detection limit (vLOD) of the Au-AIENPs-RLFIA for colorimetric and ratiomentic fluorescence detection was 0.62 ng/mL and 0.02 ng/mL, respectively, while the quantitative LOD (qLOD) for ratiomentic fluorescence mode was as low as 0.0076 ng/mL. The above results indicate that the designed Janus Au-AIENPs are promising as dual-signal output probes and hold great potential for improving flexible dual-mode detection of various targets on the LFIA platform.
期刊介绍:
Nano Today is a journal dedicated to publishing influential and innovative work in the field of nanoscience and technology. It covers a wide range of subject areas including biomaterials, materials chemistry, materials science, chemistry, bioengineering, biochemistry, genetics and molecular biology, engineering, and nanotechnology. The journal considers articles that inform readers about the latest research, breakthroughs, and topical issues in these fields. It provides comprehensive coverage through a mixture of peer-reviewed articles, research news, and information on key developments. Nano Today is abstracted and indexed in Science Citation Index, Ei Compendex, Embase, Scopus, and INSPEC.