Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia.

Tropical biomedicine Pub Date : 2024-06-01 DOI:10.47665/tb.41.2.003

A M Che Hamzah, C C Yeo, S M Puah, K H Chua, A A Rahman, S Ismail, F H Abdullah, N Othman, C H Chew

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Abstract

Staphylococcus aureus is a common bacterial pathogen known to cause various kinds of infections due to its repertoire of virulence factors. This study aimed to investigate the distribution of 19 types of virulence genes among clinical isolates of methicillin-susceptible S. aureus (MSSA) using the polymerase chain reaction. A total of 109 MSSA isolates, i.e., 63 hospital-associated (HA) and 46 community-associated (CA) were collected from Hospital Sultanah Nur Zahirah, the main tertiary hospital in Terengganu, Malaysia, from July 2016 to June 2017. The most frequent virulence genes detected were hla (78.9%, n=86) and hld (78.0%, n=85) encoding hemolysins, lukED (56.9%, n=62) encoding leukotoxin ED, followed by seb (26.6%, n=29) and sea (24.8%, n=27) encoding enterotoxins. Among 34 (31.2%) isolates carrying six or more virulence genes, only five were multidrug resistant (MDR) while the remaining isolates were susceptible. Significant associations were discovered between the hld gene with CA-MSSA (p=0.016) and the seo gene with HA-MSSA (p=0.023). However, there is no significant association between virulence genes among the different types of infection. The clinical MSSA isolates in Terengganu showed high prevalence and high diversity of virulence gene carriage.

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马来西亚丁加奴医院相关和社区相关甲氧西林金黄色葡萄球菌临床分离物中毒力基因的分布。

金黄色葡萄球菌是一种常见的细菌病原体，因其具有一系列毒力因子而导致各种感染。本研究旨在利用聚合酶链反应研究 19 种毒力基因在甲氧西林易感金黄色葡萄球菌（MSSA）临床分离株中的分布情况。2016年7月至2017年6月期间，从马来西亚丁加奴的主要三级医院苏丹努尔扎希拉医院（Hospital Sultanah Nur Zahirah）共收集到109株MSSA分离株，即63株医院相关（HA）和46株社区相关（CA）。检测到的最常见毒力基因是编码溶血素的hla（78.9%，n=86）和hld（78.0%，n=85），编码白细胞毒素ED的lukED（56.9%，n=62），其次是编码肠毒素的seb（26.6%，n=29）和sea（24.8%，n=27）。在 34 个（31.2%）携带 6 个或更多毒力基因的分离物中，只有 5 个具有多重耐药性（MDR），其余分离物均为易感性。研究发现，hld 基因与 CA-MSSA （p=0.016）、seo 基因与 HA-MSSA （p=0.023）之间存在显著关联。不过，不同感染类型的毒力基因之间并无明显关联。丁加奴的临床 MSSA 分离物显示出毒力基因携带的高流行率和高多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Tropical biomedicine

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