Sustainable cultivation of Porphyridium cruentum via agro-industrial by-products: A study on biomass and lipid enhancement

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI:10.1016/j.bcab.2024.103341

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引用次数: 0

Abstract

This study explored the cultivation of Porphyridium cruentum using beet molasses and corn steep liquor (CSL) as alternative agri-waste substrates. The objective was to enhance the biomass and lipid production of P. cruentum, known for its potential as an industrial EPA producer. Using a Box-Behnken Design (BBD) as part of the response surface methodology (RSM), we investigated the effects of beet molasses, CSL, and F/2 nutrients on the microalga's cultivation. The results demonstrated a significant influence of these factors on the final cell count and lipid content over an 8-day cultivation period. Specifically, optimal growth conditions were identified at approximately 1.78 g/L of molasses and 1.89 g/L of CSL, yielding a cell count of 12.1 × 10⁶ cells/mL and a lipid content of 24.48%. Validation experiments reaffirmed these findings, with observed results closely aligning with predicted values. Optimized media achieved also a significant higher content of carotenoids and phycobiliproteins compared to the standard media. This research highlights the potential of using agro-industrial by-products for large-scale cultivation of P. cruentum, offering a sustainable approach for enhancing lipid and pigment production.

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利用农用工业副产品可持续栽培蕨类植物：生物量和脂质增加研究

本研究探索了使用甜菜糖蜜和玉米浸出液（CSL）作为替代农业废弃物基质来培养克氏卟啉草（Porphyridium cruentum）的方法。其目的是提高 P. cruentum 的生物量和脂质产量，因为它具有生产工业 EPA 的潜力。我们使用箱-贝肯设计（BBD）作为响应面方法（RSM）的一部分，研究了甜菜糖蜜、CSL 和 F/2 营养物质对微藻培养的影响。结果表明，在 8 天的培养期内，这些因素对最终细胞数和脂质含量有显著影响。具体来说，最佳生长条件是糖蜜含量约为 1.78 克/升，CSL 含量约为 1.89 克/升，细胞数为 12.1 × 106 个/毫升，脂质含量为 24.48%。验证实验再次证实了这些发现，观察结果与预测值非常吻合。与标准培养基相比，优化培养基的类胡萝卜素和藻胆蛋白含量也显著提高。这项研究凸显了利用农用工业副产品大规模培养克氏原藻类的潜力，为提高脂质和色素产量提供了一种可持续的方法。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.