Virulence of banana wilt-causing fungal pathogen Fusarium oxysporum tropical race 4 is mediated by nitric oxide biosynthesis and accessory genes

IF 20.5 1区生物学 Q1 MICROBIOLOGY

Nature Microbiology Pub Date : 2024-08-16 DOI:10.1038/s41564-024-01779-7

Yong Zhang, Siwen Liu, Diane Mostert, Houlin Yu, Mengxia Zhuo, Gengtan Li, Cunwu Zuo, Sajeet Haridas, Katie Webster, Minhui Li, Igor V. Grigoriev, Ganjun Yi, Altus Viljoen, Chunyu Li, Li-Jun Ma

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Abstract

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most damaging plant diseases known. Foc race 1 (R1) decimated the Gros Michel-based banana (Musa acuminata) trade, and now Foc tropical race 4 (TR4) threatens global production of its replacement, the Cavendish banana. Here population genomics revealed that all Cavendish banana-infecting Foc race 4 strains share an evolutionary origin distinct from that of R1 strains. Although TR4 lacks accessory chromosomes, it contains accessory genes at the ends of some core chromosomes that are enriched for virulence and mitochondria-related functions. Meta-transcriptomics revealed the unique induction of the entire mitochondrion-localized nitric oxide (NO) biosynthesis pathway upon TR4 infection. Empirically, we confirmed the unique induction of a NO burst in TR4, suggesting that nitrosative pressure may contribute to virulence. Targeted mutagenesis demonstrated the functional importance of fungal NO production and the accessory gene SIX4 as virulence factors. Population genomics and meta-transcriptomics reveal a role of nitric oxide production and accessory genes in the virulence of the banana wilt-causing fungal pathogen Fusarium oxysporum.

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香蕉枯萎病真菌病原体 Fusarium oxysporum tropical race 4 的致病力由一氧化氮生物合成和附属基因介导

由 Fusarium oxysporum f. sp. cubense（Foc）引起的香蕉镰刀菌枯萎病是已知危害最大的植物病害之一。Foc 第 1 种（R1）使以格罗斯-米歇尔香蕉（Musa acuminata）为主的香蕉贸易锐减，现在 Foc 热带第 4 种（TR4）又威胁到其替代品卡文迪许香蕉的全球生产。群体基因组学在此揭示了所有感染卡文迪许香蕉的 Foc 第 4 种族菌株都有着不同于 R1 菌株的进化起源。虽然 TR4 缺乏附属染色体，但它在一些核心染色体的末端含有富含毒力和线粒体相关功能的附属基因。元转录组学揭示了 TR4 感染后对整个线粒体定位的一氧化氮（NO）生物合成途径的独特诱导作用。根据经验，我们证实了 TR4 对一氧化氮爆发的独特诱导作用，这表明亚硝酸压力可能有助于提高病毒的毒性。定向诱变证明了真菌一氧化氮产生和附属基因 SIX4 作为毒力因子的功能重要性。

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来源期刊

Nature Microbiology Immunology and Microbiology-Microbiology

CiteScore

44.40

自引率

1.10%

发文量

226

期刊介绍： Nature Microbiology aims to cover a comprehensive range of topics related to microorganisms. This includes: Evolution: The journal is interested in exploring the evolutionary aspects of microorganisms. This may include research on their genetic diversity, adaptation, and speciation over time. Physiology and cell biology: Nature Microbiology seeks to understand the functions and characteristics of microorganisms at the cellular and physiological levels. This may involve studying their metabolism, growth patterns, and cellular processes. Interactions: The journal focuses on the interactions microorganisms have with each other, as well as their interactions with hosts or the environment. This encompasses investigations into microbial communities, symbiotic relationships, and microbial responses to different environments. Societal significance: Nature Microbiology recognizes the societal impact of microorganisms and welcomes studies that explore their practical applications. This may include research on microbial diseases, biotechnology, or environmental remediation. In summary, Nature Microbiology is interested in research related to the evolution, physiology and cell biology of microorganisms, their interactions, and their societal relevance.