Teck Wei Lim , Renee Lay Hong Lim , Liew Phing Pui , Chin Ping Tan , Chun Wai Ho
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引用次数: 0
Abstract
Red dragon fruit (RDF, Hylocereus polyrhizus) is high in betacyanins content but is understudied for its antioxidant mechanisms in liver cells. The present study aimed to investigate the antioxidant mechanisms of the stabilised betacyanins in a novel RDF functional drink, Improved-FRDFD-dH2O (produced from mild, sustainable approaches) using HepG2 cell line. Results revealed the betacyanins of Improved-FRDFD-dH2O (12.5% and 25%, v/v) showed the highest direct scavenging effect on intracellular ROS from 75.39% to <31%. The antioxidant enzymes (SOD, CAT and GPx) activities in HepG2 cells were remarkably increased, with 12.5% and 25% Improved-FRDFD-dH2O demonstrating the greatest enhancing effects. RT-qPCR proved the betacyanins of Improved-FRDFD-dH2O possessed indirect antioxidant mechanisms, where the gene expressions associated with Nrf2-ARE pathway were upregulated by all the tested sample concentrations (highest fold: 6.82). Overall, the present findings highlighted the RDF's potential in functional product development with betacyanins as the prominent bioactive compound to provide direct and indirect antioxidative and hepatoprotective activities.
期刊介绍:
Sustainable Materials and Technologies (SM&T), an international, cross-disciplinary, fully open access journal published by Elsevier, focuses on original full-length research articles and reviews. It covers applied or fundamental science of nano-, micro-, meso-, and macro-scale aspects of materials and technologies for sustainable development. SM&T gives special attention to contributions that bridge the knowledge gap between materials and system designs.