RpedOBP1 plays key roles in aggregation pheromones reception of the Riptortus pedestris

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2024-08-05 DOI:10.1016/j.pestbp.2024.106073

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Abstract

Riptortus pedestris (Hemiptera: Alydidae) is a notable soybean pest, with diapause and non-diapause individuals showing different sensitivities to aggregation pheromones. This study aimed to investigate how R. pedestris detects aggregation pheromones through electroantennogram (EAG) and behavioral experiments, transcriptome sequencing and qRT-PCR, as well as competitive fluorescence-binding assay. Results indicated that diapausing females and males of R. pedestris exhibited a heightened EAG response and were more attracted to the aggregation pheromone components compared to their non-diapause counterparts. Transcriptome sequencing and qRT-PCR analyses revealed significantly higher expression of RpedOBP1 in the antennae of diapause females and males compared to non-diapausing R. pedestris. The competitive fluorescence-binding assay demonstrated that RpedOBP1 displayed the strongest binding affinity to E2HE2H, suggesting its crucial role in recognizing the aggregation pheromone. These findings have the potential to inform the development of integrated pest management strategies utilizing behavioral approaches for bean bug control.

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RpedOBP1 在栉水母的聚集信息素接收过程中发挥关键作用

Riptortus pedestris（半翅目：Alydidae）是一种显著的大豆害虫，其休眠期和非休眠期个体对聚集信息素表现出不同的敏感性。本研究旨在通过电触角图谱（EAG）和行为实验、转录组测序和 qRT-PCR 以及竞争性荧光结合试验，研究 R. pedestris 如何检测聚集信息素。结果表明，与非停歇期的雌性和雄性相比，停歇期的雌性和雄性表现出更高的 EAG 反应，对聚集信息素成分的吸引力更大。转录组测序和 qRT-PCR 分析表明，与非停歇期相比，停歇期雌虫和雄虫触角中 RpedOBP1 的表达量明显更高。竞争性荧光结合试验表明，RpedOBP1与E2HE2H的结合亲和力最强，这表明它在识别聚集信息素方面起着关键作用。这些发现有可能为利用行为方法控制豆角虫的害虫综合治理战略的开发提供信息。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.

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