RACK1 links phyB and BES1 to coordinate brassinosteroid-dependent root meristem development

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-08-16 DOI:10.1111/nph.20055

Wei Zhu, Yajuan Fu, Hua Zhou, Yeling Zhou, Dayan Zhang, Yuzhu Wang, Yujing Su, Zhiyong Li, Jiansheng Liang

{"title":"RACK1 links phyB and BES1 to coordinate brassinosteroid-dependent root meristem development","authors":"Wei Zhu, Yajuan Fu, Hua Zhou, Yeling Zhou, Dayan Zhang, Yuzhu Wang, Yujing Su, Zhiyong Li, Jiansheng Liang","doi":"10.1111/nph.20055","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n \n </p><ul>\n \n \n <li>Light and brassinosteroids (BR) are indispensable for plant growth and control cell division in the apical meristem. However, how external light signals cooperate with internal brassinosteroids to program root meristem development remains elusive.</li>\n \n \n <li>We reveal that the photoreceptor phytochrome B (phyB) guides the scaffold protein RACK1 to coordinate BR signaling for maintaining root meristematic activity. phyB and RACK1 promote early root meristem development.</li>\n \n \n <li>Mechanistically, RACK1 could reinforce the phyB–SPA1 association by interacting with both phyB and SPA1, which indirectly affects COP1-dependent RACK1 degradation, resulting in the accumulation of RACK1 in roots. Subsequently, RACK1 interacts with BES1 to repress its DNA-binding activity toward the target gene <i>CYCD3;1</i>, leading to the release of BES1-mediated inhibition of <i>CYCD3;1</i> transcription, and hence the promotion of root meristem development.</li>\n \n \n <li>Our study provides mechanistic insights into the regulation of root meristem development by combination of light and phytohormones signals through the photoreceptors and scaffold proteins.</li>\n </ul>\n \n </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":8.3000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/nph.20055","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Light and brassinosteroids (BR) are indispensable for plant growth and control cell division in the apical meristem. However, how external light signals cooperate with internal brassinosteroids to program root meristem development remains elusive.
We reveal that the photoreceptor phytochrome B (phyB) guides the scaffold protein RACK1 to coordinate BR signaling for maintaining root meristematic activity. phyB and RACK1 promote early root meristem development.
Mechanistically, RACK1 could reinforce the phyB–SPA1 association by interacting with both phyB and SPA1, which indirectly affects COP1-dependent RACK1 degradation, resulting in the accumulation of RACK1 in roots. Subsequently, RACK1 interacts with BES1 to repress its DNA-binding activity toward the target gene CYCD3;1, leading to the release of BES1-mediated inhibition of CYCD3;1 transcription, and hence the promotion of root meristem development.
Our study provides mechanistic insights into the regulation of root meristem development by combination of light and phytohormones signals through the photoreceptors and scaffold proteins.

查看原文本刊更多论文

RACK1 连接 phyB 和 BES1，协调黄铜类固醇依赖性根分生组织的发育。

光和黄铜类固醇（BR）是植物生长和控制顶端分生组织细胞分裂所不可或缺的。然而，外部光信号如何与内部铜绿素类固醇合作来控制根分生组织的发育仍然是个谜。我们揭示了光感受器植物色素 B（phyB）引导支架蛋白 RACK1 协调 BR 信号以维持根分生组织活性。从机理上讲，RACK1 可通过与 phyB 和 SPA1 相互作用来加强 phyB-SPA1 的关联，从而间接影响 COP1 依赖性的 RACK1 降解，导致 RACK1 在根中积累。随后，RACK1 与 BES1 相互作用，抑制其对靶基因 CYCD3;1 的 DNA 结合活性，从而释放 BES1 介导的对 CYCD3;1 转录的抑制，进而促进根分生组织的发育。我们的研究从机理上揭示了光和植物激素信号通过光感受器和支架蛋白共同调控根分生组织发育的过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.