Dynamics of the thymic transcriptome at stages of acute thymic involution in Japanese Black calves with a poor prognosis

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Ryogo Furukawa, Shoichi Wakitani, Risako Kawabata, Masahiro Yasuda
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Abstract

Transcriptome analysis was performed on the thymus of Japanese Black calves that were necropsied due to poor prognosis, to characterize changes associated with acute thymic involution. Gene expression profiles obtained by DNA microarray analysis of eight calf thymuses were classified into three patterns that correlated with the histopathological stage of acute thymic involution. Using principal component analysis, the first principal component of the global gene expression levels in the calf thymus was associated with the stage of acute thymic involution, suggesting that histopathological changes greatly influence the gene expression profile. Gene ontology enrichment analysis revealed that genes related to cell proliferation, wound healing, and inflammatory responses were the main contributors to the first principal component. Real-time RT-PCR showed that the thymus had lower expression of PCNA, KIFC1, and HES6, and higher expression of SYNPO2, PDGFRB, and TWIST1 during acute thymic involution. Immunohistochemistry demonstrated a decrease in the rate of Ki67-positive cells in the thymic cortex during the late stage of acute thymic involution. The rate of cleaved caspase-1-positive cells increased in the thymic cortex at an earlier stage than the increase in the rate of cleaved caspase-3-positive cells. Vimentin, which was almost absent in the non-involuted thymic cortex, appeared in the thymic cortex during acute thymic involution. These results suggest that in farmed calves with a poor prognosis, inflammatory responses and impaired thymocyte proliferation are primarily involved in acute thymic involution.

预后不良的日本黑犊牛在急性胸腺萎缩阶段的胸腺转录组动态。
我们对因预后不良而死亡的日本黑犊牛胸腺进行了转录组分析,以确定与急性胸腺萎缩相关的变化特征。通过对 8 头犊牛胸腺进行 DNA 微阵列分析获得的基因表达谱分为三种模式,它们与急性胸腺萎缩的组织病理学阶段相关。通过主成分分析,小牛胸腺全局基因表达水平的第一个主成分与急性胸腺萎缩的阶段有关,这表明组织病理学变化对基因表达谱有很大影响。基因本体富集分析表明,与细胞增殖、伤口愈合和炎症反应相关的基因是第一个主成分的主要贡献者。实时 RT-PCR 显示,在胸腺急性内陷期,胸腺中 PCNA、KIFC1 和 HES6 的表达较低,而 SYNPO2、PDGFRB 和 TWIST1 的表达较高。免疫组化显示,在急性胸腺萎缩晚期,胸腺皮质中 Ki67 阳性细胞的比率有所下降。胸腺皮质中已裂解的 Caspase-1 阳性细胞比率的增加早于已裂解的 Caspase-3 阳性细胞比率的增加。波形蛋白在未内陷的胸腺皮质中几乎不存在,但在胸腺急性内陷期出现在胸腺皮质中。这些结果表明,在预后不良的养殖犊牛中,炎症反应和胸腺细胞增殖受损是急性胸腺萎缩的主要原因。
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来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
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