Shaohua Pi, Razieh Ganjee, Lingyun Wang, Riley K Arbuckle, Chengcheng Zhao, Jose A Sahel, Bingjie Wang, Yuanyuan Chen
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引用次数: 0
Abstract
This study introduces an innovative optical coherence tomography (OCT) imaging system dedicated to high-throughput screening applications using ex vivo tissue culture. Leveraging OCT's non-invasive, high-resolution capabilities, the system is equipped with a custom-designed motorized platform and tissue detection ability for automated, successive imaging across samples. Transformer-based deep-learning segmentation algorithms further ensure robust, consistent, and efficient readouts meeting the standards for screening assays. Validated using retinal explant cultures from a mouse model of retinal degeneration, the system provides robust, rapid, reliable, unbiased, and comprehensive readouts of tissue response to treatments. This fully automated OCT-based system marks a significant advancement in tissue screening, promising to transform drug discovery, as well as other relevant research fields.
本研究介绍了一种创新的光学相干断层扫描（OCT）成像系统，专用于利用体内外组织培养进行高通量筛选应用。利用 OCT 的无创、高分辨率功能，该系统配备了一个定制设计的电动平台和组织检测能力，可对样品进行自动、连续成像。基于变压器的深度学习分割算法进一步确保了读数的稳健性、一致性和高效性，符合筛选检测的标准。通过使用视网膜变性小鼠模型的视网膜外植体培养物进行验证，该系统可提供稳健、快速、可靠、无偏见和全面的组织对治疗反应的读数。这种基于 OCT 的全自动系统标志着组织筛选领域的重大进步，有望改变药物发现及其他相关研究领域。
期刊介绍：
The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community.
Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.
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