Brazil nuts potential: effects on lipid peroxidation and heart health in nephrectomized rats.

Q2 Medicine
Journal of Complementary and Integrative Medicine Pub Date : 2024-08-16 eCollection Date: 2024-09-01 DOI:10.1515/jcim-2024-0123
Agatha Cristie Menezes, Michele Lima Brito, Patricia Pereira Almeida, Beatriz Oliveira Da Cruz, Nathalia da Silva Costa, Aline D'Avila Pereira, Cecilia Castañon, Viviane Alexandre Nunes Degani, Ludmila Ferreira Medeiros de França Cardozo, D'Angelo Carlo Magliano, Milena Barcza Stockler-Pinto
{"title":"Brazil nuts potential: effects on lipid peroxidation and heart health in nephrectomized rats.","authors":"Agatha Cristie Menezes, Michele Lima Brito, Patricia Pereira Almeida, Beatriz Oliveira Da Cruz, Nathalia da Silva Costa, Aline D'Avila Pereira, Cecilia Castañon, Viviane Alexandre Nunes Degani, Ludmila Ferreira Medeiros de França Cardozo, D'Angelo Carlo Magliano, Milena Barcza Stockler-Pinto","doi":"10.1515/jcim-2024-0123","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of a Brazil nut-enriched diet on the wall thickness and the left ventricular chamber diameter of the heart, and lipid peroxidation in a CKD-induced model.</p><p><strong>Methods: </strong>Male <i>Wistar</i> rats at 12 weeks of age were divided into two groups (n=16/group): the Nx group, which underwent 5/6 nephrectomy, and the Sham group, as a control. After 5 weeks, the groups were subdivided according to diet (n=8/group): the Nx and Sham groups received a control diet; the Nx5% and Sham5% groups received a diet enriched with 5 % Brazil nuts for 8 weeks. The left ventricular thickening and chamber diameter were determined. Plasma biochemical parameters were evaluated. Analysis of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activity was performed in the plasma and the left ventricle (LV). LV mRNA expression of nuclear factor-kappa B (NF-κB) and nuclear factor erythroid 2-related factor 2 (Nrf2) was evaluated by reverse transcription-polymerase chain reaction.</p><p><strong>Results: </strong>The Nx5% group showed a remodeled LV wall with decreased thickness compared to the Nx group (p=0.016). Furthermore, LV TBARS concentration was reduced in the Nx5% group (p=0.0064). In addition, the Nx5% group showed an increase in plasma GPx activity (p=0.0431). No significant results were found concerning the LV mRNA expression of NF-κB and Nrf2 genes.</p><p><strong>Conclusions: </strong>A Brazil nut-enriched diet decreased LV thickness and LV TBARS concentration and increased GPx activity in a 5/6 nephrectomy experimental model, making it a promising adjuvant therapy to improve antioxidant status and cardiovascular outcomes in chronic kidney disease.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"340-348"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Complementary and Integrative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/jcim-2024-0123","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Objectives: To investigate the effects of a Brazil nut-enriched diet on the wall thickness and the left ventricular chamber diameter of the heart, and lipid peroxidation in a CKD-induced model.

Methods: Male Wistar rats at 12 weeks of age were divided into two groups (n=16/group): the Nx group, which underwent 5/6 nephrectomy, and the Sham group, as a control. After 5 weeks, the groups were subdivided according to diet (n=8/group): the Nx and Sham groups received a control diet; the Nx5% and Sham5% groups received a diet enriched with 5 % Brazil nuts for 8 weeks. The left ventricular thickening and chamber diameter were determined. Plasma biochemical parameters were evaluated. Analysis of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activity was performed in the plasma and the left ventricle (LV). LV mRNA expression of nuclear factor-kappa B (NF-κB) and nuclear factor erythroid 2-related factor 2 (Nrf2) was evaluated by reverse transcription-polymerase chain reaction.

Results: The Nx5% group showed a remodeled LV wall with decreased thickness compared to the Nx group (p=0.016). Furthermore, LV TBARS concentration was reduced in the Nx5% group (p=0.0064). In addition, the Nx5% group showed an increase in plasma GPx activity (p=0.0431). No significant results were found concerning the LV mRNA expression of NF-κB and Nrf2 genes.

Conclusions: A Brazil nut-enriched diet decreased LV thickness and LV TBARS concentration and increased GPx activity in a 5/6 nephrectomy experimental model, making it a promising adjuvant therapy to improve antioxidant status and cardiovascular outcomes in chronic kidney disease.

巴西坚果的潜力:对肾切除大鼠脂质过氧化和心脏健康的影响。
目的研究富含巴西坚果的饮食对慢性肾功能衰竭模型中心脏壁厚度和左心室腔直径以及脂质过氧化的影响:将 12 周大的雄性 Wistar 大鼠分为两组(n=16/组):Nx 组(进行 5/6 肾切除)和 Sham 组(作为对照)。5 周后,根据饮食(n=8/组)对各组进行细分:Nx 组和 Sham 组接受对照饮食;Nx5% 组和 Sham5% 组接受富含 5% 巴西坚果的饮食,为期 8 周。测定左心室增厚和心腔直径。对血浆生化指标进行了评估。对血浆和左心室的硫代巴比妥酸活性物质(TBARS)和抗氧化酶活性进行了分析。通过反转录聚合酶链反应评估了核因子卡巴B(NF-κB)和核因子红细胞2相关因子2(Nrf2)在左心室中的mRNA表达:结果:与Nx组相比,Nx5%组左心室壁重塑,厚度减少(p=0.016)。此外,Nx5% 组的左心室 TBARS 浓度降低(p=0.0064)。此外,Nx5% 组显示血浆 GPx 活性增加(p=0.0431)。NF-κB和Nrf2基因在左心室mRNA表达方面没有发现明显结果:结论:在5/6肾切除术实验模型中,巴西坚果富含饮食可降低左心室厚度和左心室TBARS浓度,提高GPx活性,是一种很有前景的辅助疗法,可改善慢性肾病患者的抗氧化状态和心血管预后。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Complementary and Integrative Medicine
Journal of Complementary and Integrative Medicine Medicine-Complementary and Alternative Medicine
CiteScore
3.10
自引率
0.00%
发文量
51
期刊介绍: Journal of Complementary and Integrative Medicine (JCIM) focuses on evidence concerning the efficacy and safety of complementary medical (CM) whole systems, practices, interventions and natural health products, including herbal and traditional medicines. The journal is edited by Ed Lui of the University of Western Ontario. Topics: -Quality, efficacy, and safety of natural health products, dietary supplements, traditional medicines and their synthetic duplicates -Efficacy and safety of complementary therapies -Evidence-based medicine and practice, including evidence of traditional use -Curriculum development, educational system and competency of complementary health programs -Methodologies on research and evaluation of traditional medicines and herbal products -Integrative medicine: basic and clinical research and practice -Innovation in CAM Curriculum -Educational Material Design
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信