Enzyme-aided amplification strategy for sensitive detection of methamphetamine based on fluorescence aptamer sensor

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2024-08-16 DOI:10.1007/s44211-024-00648-x

Zheyu Wang, Yandan Wang, Yishuo Tong, Weifen Niu

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Abstract

Methamphetamine (METH) abuse poses a serious risk to human health and social stability. It is critical to develop sensitive and selective methods for detecting METH. Here, we develop a fluorescence aptamer sensor to detect METH based on DNA exonuclease III (Exo III), graphene oxide (GO), and FAM-labeled aptamer. First, the sensor used GO’s strong binding capacity to adsorb and quench the fluorescence of the aptamer attached to GO surface. When METH was added to the system, the formation of stable complex for aptamer and METH dissociated from the surface of GO, leading to a fluorescence restoration. Then, the fluorescence signal was further amplified by using Exo III to liberate target METH for cyclic hybridization. And the gel electrophoresis experiment further verified the reliability of this strategy. This aptamer sensor exhibited a low detection limit (0.52 nM) and excellent selectivity under optimal conditions. Notably, this sensor has been successfully validated in the detection of METH in urine and saliva samples, exhibited commendable recovery (94.00–104.65%). Its benefits include facile, sensitive, and rapid. Expected to be used in practical METH detection.

Graphical abstract

Abstract Image

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基于荧光适配体传感器灵敏检测甲基苯丙胺的酶辅助放大策略。

甲基苯丙胺（METH）滥用对人类健康和社会稳定构成严重威胁。开发灵敏且具有选择性的甲基苯丙胺检测方法至关重要。在此，我们以 DNA 外切酶 III（Exo III）、氧化石墨烯（GO）和 FAM 标记的适配体为基础，开发了一种检测 METH 的荧光适配体传感器。首先，该传感器利用 GO 的强结合能力吸附并淬灭附着在 GO 表面的适配体的荧光。当向系统中加入 METH 时，贴体与 METH 形成的稳定复合物从 GO 表面解离，从而恢复荧光。然后，利用 Exo III 进一步放大荧光信号，释放出目标 METH 进行循环杂交。凝胶电泳实验进一步验证了这一策略的可靠性。在最佳条件下，该传感器的检测限低（0.52 nM），选择性好。值得注意的是，该传感器已成功用于检测尿液和唾液样本中的甲基苯丙胺，回收率高达 94.00-104.65%。其优点包括简便、灵敏和快速。有望用于实际的 METH 检测。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.