Evaluating the efficacy of three Y-STRs commercial kits in degraded skeletal remains

IF 1.9 4区医学 Q2 MEDICINE, LEGAL

Science & Justice Pub Date : 2024-07-31 DOI:10.1016/j.scijus.2024.07.007

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引用次数: 0

Abstract

Y chromosome short tandem repeats (Y-STRs) typing is a useful tool in scenarios such as mass graves analysis or disaster victim identification and has become a routine analysis in many laboratories. Not many comparisons have been performed with the currently available commercial kits, much less with degraded skeletal remains. This research aims to evaluate the performance of three commercial Y-STR kits: Yfiler™ Plus, PowerPlex® Y23, and Investigator® Argus Y-28 in 63 degraded skeletal remains from mass graves. PowerPlex® Y23 yields more reportable markers and twice the RFU on average, while Yfiler™ Plus and Investigator® Argus Y-28 exhibited a similar behaviour. Additionally, Argus Y-28, which has not been tested with this kind of samples in literature before, showed a good performance. Finally, a predictive model was attempted to be developed from quantification and autosomal STR data. However, no acceptable model could be obtained. Nevertheless, good Y-STR typing results may be expected if at least 50 pg DNA input is used or 13 autosomal markers were previously obtained.

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评估三种 Y-STRs 商用试剂盒在退化骸骨中的功效

Y 染色体短串联重复序列（Y-STR）分型是乱葬坑分析或灾难受害者身份鉴定等情况下的有用工具，已成为许多实验室的常规分析方法。与目前可用的商业试剂盒进行比较的情况并不多，更不用说与退化的骸骨进行比较了。本研究旨在评估三种商用 Y-STR 检测试剂盒的性能：Yfiler™ Plus、PowerPlex® Y23 和 Investigator® Argus Y-28 在 63 具来自乱葬坑的退化骸骨中的表现。PowerPlex® Y23产生的可报告标记物更多，平均RFU是Yfiler™ Plus和Investigator® Argus Y-28的两倍，而Yfiler™ Plus和Investigator® Argus Y-28的表现类似。此外，Argus Y-28 也表现出了良好的性能，而之前的文献中并没有用这种样本进行过测试。最后，尝试从定量和常染色体 STR 数据中建立一个预测模型。然而，并没有获得可接受的模型。不过，如果使用至少 50 pg DNA 输入或之前已获得 13 个常染色体标记，则有望获得良好的 Y-STR 分型结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science & Justice 医学-病理学

CiteScore

4.20

自引率

15.80%

发文量

审稿时长

81 days

期刊介绍： Science & Justice provides a forum to promote communication and publication of original articles, reviews and correspondence on subjects that spark debates within the Forensic Science Community and the criminal justice sector. The journal provides a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. Science & Justice is published six times a year, and will be of interest primarily to practising forensic scientists and their colleagues in related fields. It is chiefly concerned with the publication of formal scientific papers, in keeping with its international learned status, but will not accept any article describing experimentation on animals which does not meet strict ethical standards. Promote communication and informed debate within the Forensic Science Community and the criminal justice sector. To promote the publication of learned and original research findings from all areas of the forensic sciences and by so doing to advance the profession. To promote the publication of case based material by way of case reviews. To promote the publication of conference proceedings which are of interest to the forensic science community. To provide a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. To appeal to all those with an interest in the forensic sciences.