A gene cassette Vd276-280 in Verticillium dahliae contains two genes that affect melanized microsclerotium formation and virulence.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-08-15 DOI:10.1094/PHYTO-11-23-0426-R

Tao Liu, Haonan Yu, Jun Qin, Wenjing Shang, Jieyin Chen, Krishna V Subbarao, Xiaoping Hu

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引用次数: 0

Abstract

Verticillium dahliae is a soilborne phytopathogenic fungus causing Verticillium wilt on hundreds of plant species. Several sequenced genomes of V. dahliae are available, but functional characterization of most genes has just begun. Based on our previous comparison of the transcriptome from the wild-type and ΔVdCf2 strains, a significant upregulation of the gene cassette, Vd276-280, in the ΔVdCf2 strain was observed. In this study, the functional characterization of the Vd276-280 gene cassette was performed. Agrobacterium-mediated knockout of this gene cassette in V. dahliae significantly inhibited conidiation, melanized microsclerotium formation in the mutant strains, and their virulence towards cotton. Furthermore, deletion of individual genes in the Vd276-280 gene cassette identified that the disruption of VDAG_07276 and VDAG_07280 delayed microsclerotium formation, inhibited conidiation, and reduced virulence towards cotton. Our data suggest that VDAG_07276 and VDAG_07280 in the Vd276-280 gene cassette mainly act as positive regulators of development and virulence in V. dahliae.

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大丽轮枝菌（Verticillium dahliae）中的一个基因盒 Vd276-280 包含两个影响黑色素化小硬壳形成和毒力的基因。

大丽轮枝菌（Verticillium dahliae）是一种通过土壤传播的植物病原真菌，可导致数百种植物发生轮枝枯萎病。目前已有多个大丽轮枝菌基因组测序结果，但大多数基因的功能表征才刚刚开始。根据我们之前对野生型菌株和 ΔVdCf2 菌株转录组的比较，在 ΔVdCf2 菌株中观察到基因盒 Vd276-280 的显著上调。本研究对 Vd276-280 基因盒进行了功能鉴定。通过农杆菌介导敲除大丽花病毒中的这一基因盒，可显著抑制突变株的分生、黑色化小硬壳的形成及其对棉花的毒力。此外，删除 Vd276-280 基因盒中的单个基因后发现，VDAG_07276 和 VDAG_07280 基因的缺失会延迟小圆孢子囊的形成，抑制分生，并降低对棉花的毒力。我们的数据表明，Vd276-280 基因盒中的 VDAG_07276 和 VDAG_07280 主要作为大丽花病毒发育和毒力的正调控因子。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.