Emma Johnson, Talbot Kinney, Hannah Luellen, Rhiannan Amerud, Daysha R Anderson, Marie Anderson, Arnelyn Mae Andres, Rameel Arshad, Kylie Babin-Howard, Dede G Barrigah, Addison Beauregard, Leah Beise, Nolan Christofferson, Elijah L David, Luke DeWaard, Maya Diaz, Lily Donner, Natalie Ehlinger, Diellza Elmazi, Riley Engelhardt, Tamkanat Farheen, Mark M Figueroa, Soren Flaten, Madison Frush, Elizabeth Gonzalez, Jaylen Goolsby, Estefania Guzman, Logan Hanson, John Hejl, Jackson Heuschel, Brianna Higgins, Brylee Hoeppner, Daijah Hollins, Josette Knutson, Rachel Lemont, Mia Lopez, Samantha Martin, Trinity May, Abby McDade, Nearyroth Men, Ellie Meyer, Caroline R Mickle, Sebastian Mireles, Avery Mize, Jaiden Neuhaus, April Ost, Sarah Piane, Makenzie Pianovski, Aliya Rangel, Jessica Reyes, Alexandra Ruttenberg, Jacob D Sachs, Brandon Schluns, Nicholas Schroeder, Peighton R Skrobot, Cylie Smith, Sydney Stout, Andrew Valenzuela, Kaiden P Vinavich, Amber K Weaver, Michael Yager, Jose Zaragoza, Gabriela Zawadzki, Weam El Rahmany, Nicole L Scheuermann, Hemin P Shah, Kayla L Bieser, Paula Croonquist, Olivier Devergne, Elizabeth E Taylor, Jacqueline K Wittke-Thompson, Jacob D Kagey, Stephanie Toering Peters
{"title":"Genetic Mapping of <i>prod <sup>E.3.3</sup></i> , a New Lethal Allele of <i>prod</i>.","authors":"Emma Johnson, Talbot Kinney, Hannah Luellen, Rhiannan Amerud, Daysha R Anderson, Marie Anderson, Arnelyn Mae Andres, Rameel Arshad, Kylie Babin-Howard, Dede G Barrigah, Addison Beauregard, Leah Beise, Nolan Christofferson, Elijah L David, Luke DeWaard, Maya Diaz, Lily Donner, Natalie Ehlinger, Diellza Elmazi, Riley Engelhardt, Tamkanat Farheen, Mark M Figueroa, Soren Flaten, Madison Frush, Elizabeth Gonzalez, Jaylen Goolsby, Estefania Guzman, Logan Hanson, John Hejl, Jackson Heuschel, Brianna Higgins, Brylee Hoeppner, Daijah Hollins, Josette Knutson, Rachel Lemont, Mia Lopez, Samantha Martin, Trinity May, Abby McDade, Nearyroth Men, Ellie Meyer, Caroline R Mickle, Sebastian Mireles, Avery Mize, Jaiden Neuhaus, April Ost, Sarah Piane, Makenzie Pianovski, Aliya Rangel, Jessica Reyes, Alexandra Ruttenberg, Jacob D Sachs, Brandon Schluns, Nicholas Schroeder, Peighton R Skrobot, Cylie Smith, Sydney Stout, Andrew Valenzuela, Kaiden P Vinavich, Amber K Weaver, Michael Yager, Jose Zaragoza, Gabriela Zawadzki, Weam El Rahmany, Nicole L Scheuermann, Hemin P Shah, Kayla L Bieser, Paula Croonquist, Olivier Devergne, Elizabeth E Taylor, Jacqueline K Wittke-Thompson, Jacob D Kagey, Stephanie Toering Peters","doi":"10.17912/micropub.biology.001236","DOIUrl":null,"url":null,"abstract":"<p><p>The <i>E.3.3</i> mutation was generated in a Flp/FRT EMS screen for conditional mutations that cause growth and developmental defects in a genetic background that blocks apoptosis. The mutations were conditional, based on the <i>Dark <sup>82</sup></i> allele being present on the starting chromosome, and blocking canonical apoptosis in a homozygous state. The <i>E.3.3</i> mosaic eyes exhibit defects in eye development including patches of rough eye and irregular surface structure. Whole Genome Sequencing and complementation mapping revealed <i>E.3.3</i> as an allele of <i>prod</i> . Prod is a DNA-binding protein that binds satellite repeats and is involved in chromocenter formation during mitosis. Here we present a novel allele of <i>prod</i> , <i>prod <sup>E.3.3</sup></i> , that disrupts the functional region of the Prod protein resulting in disruption of typical eye structure, likely due to disruption of chromatid separation during development.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320118/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001236","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The E.3.3 mutation was generated in a Flp/FRT EMS screen for conditional mutations that cause growth and developmental defects in a genetic background that blocks apoptosis. The mutations were conditional, based on the Dark 82 allele being present on the starting chromosome, and blocking canonical apoptosis in a homozygous state. The E.3.3 mosaic eyes exhibit defects in eye development including patches of rough eye and irregular surface structure. Whole Genome Sequencing and complementation mapping revealed E.3.3 as an allele of prod . Prod is a DNA-binding protein that binds satellite repeats and is involved in chromocenter formation during mitosis. Here we present a novel allele of prod , prod E.3.3 , that disrupts the functional region of the Prod protein resulting in disruption of typical eye structure, likely due to disruption of chromatid separation during development.
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