An automated method for thrombocyte counting in capillary microsamples

IF 2.2 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2024-08-14 DOI:10.1111/ijlh.14354

Caroline Vasard Boesen, Vibeke Staun Christensen, Klaus Rosenkilde Jensen, Anja Reinert Hansen, Claus Vinter Bødker Hviid

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引用次数: 0

Abstract

Introduction

We aimed to develop an automated, low-volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.

Methods

Microsamples were prepared by resuspension of 50 μL blood in 300 μL DCL CellPack. Thrombocyte counting was done in the impedance (PLT-I) and fluorescence (PLT-F) channels. The imprecision and bias was evaluated in >394 microsamples from adult blood. Preanalytical factors (skin-piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.

Results

For PLT-F, the imprecision was 1.1%–3.7%, and the bias was 10.1% (95% CI: 8.8–11.3). After skin-piercing, the bias was 8.1% (95% CI: 5.6–10.6) and the imprecision 1.9% (95% CI: 1.3–2.5). Thrombocyte counts kept stable after 4 h at room temperature (94.8% [95% CI: 93.2–96.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8–8.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: −8.4–34.4) in the PLT-F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.

Conclusion

The automated microsample method offers a low-volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.

Abstract Image

查看原文本刊更多论文

毛细管微型样本中血小板计数的自动方法。

简介：我们的目的是利用 Sysmex 预稀释（PD）模式开发一种自动、低容量的毛细管血液血小板计数方法：我们的目标是利用 Sysmex 预稀释（PD）模式开发一种自动、低容量的毛细管血液血小板计数方法：方法：用 300 μL DCL CellPack 重悬 50 μL 血液制备微量样本。在阻抗（PLT-I）和荧光（PLT-F）通道中进行血小板计数。在大于 394 个成人血液微量样本中对不精确度和偏差进行了评估。对分析前因素（皮肤穿刺、储存和气管系统运输）进行了评估，并对儿科微量样本进行了比较研究。对分析质量和周转时间的改善进行了研究：对于 PLT-F，不精确度为 1.1%-3.7%，偏差为 10.1%（95% CI：8.8-11.3）。皮肤穿刺后，偏差为 8.1%（95% CI：5.6-10.6），不精确度为 1.9%（95% CI：1.3-2.5）。室温下 4 小时后（94.8% [95% CI：93.2-96.4]）和气管运输后[6.7% (95% CI：4.8-8.6)]，血小板计数保持稳定。在 PLT-F 通道中，儿科微量样本的 PD 模式偏差为 13.0%（95% CI：-8.4-34.4）。与现有的手动血小板计数方法相比，自动方法的不精确度要低得多，而且减少了周转时间：结论：自动微样本方法为测量血小板提供了一种低容量的替代方法。结论：自动微量样本法为测量血小板提供了一种低容量的替代方法，该方法似乎也适用于儿科样本。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.