The pentatricopeptide repeat protein DG1 promotes the transition to bilateral symmetry during Arabidopsis embryogenesis through GUN1-mediated plastid signals

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-08-14 DOI:10.1111/nph.20056

Yajie Li, Yiqiong Liu, Guiping Ran, Yue Yu, Yifan Zhou, Yuxian Zhu, Yujuan Du, Limin Pi

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引用次数: 0

Abstract

During Arabidopsis embryogenesis, the transition of the embryo's symmetry from radial to bilateral between the globular and heart stage is a crucial event, involving the formation of cotyledon primordia and concurrently the establishment of a shoot apical meristem (SAM). However, a coherent framework of how this transition is achieved remains to be elucidated.
In this study, we investigated the function of DELAYED GREENING 1 (DG1) in Arabidopsis embryogenesis using a newly identified dg1-3 mutant. The absence of chloroplast-localized DG1 in the mutants led to embryos being arrested at the globular or heart stage, accompanied by an expansion of WUSCHEL (WUS) and SHOOT MERISTEMLESS (STM) expression. This finding pinpoints the essential role of DG1 in regulating the transition to bilateral symmetry. Furthermore, we showed that this regulation of DG1 may not depend on its role in plastid RNA editing.
Nevertheless, we demonstrated that the DG1 function in establishing bilateral symmetry is genetically mediated by GENOMES UNCOUPLED 1 (GUN1), which represses the transition process in dg1-3 embryos.
Collectively, our results reveal that DG1 functionally antagonizes GUN1 to promote the transition of the Arabidopsis embryo's symmetry from radial to bilateral and highlight the role of plastid signals in regulating pattern formation during plant embryogenesis.

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拟南芥胚胎发生过程中，五肽重复蛋白 DG1 通过 GUN1 介导的质体信号促进向两侧对称过渡。

在拟南芥胚胎发生过程中，胚胎的对称性在球心期和心脏期之间从辐射对称过渡到两侧对称是一个关键事件，涉及子叶原基的形成以及同时嫩枝顶端分生组织（SAM）的建立。然而，如何实现这一转变的连贯框架仍有待阐明。在本研究中，我们利用新发现的 dg1-3 突变体研究了延迟绿化 1（DG1）在拟南芥胚胎发生过程中的功能。突变体中叶绿体定位的 DG1 的缺失导致胚胎停滞在球心期或心脏期，同时 WUSCHEL（WUS）和 SHOOT MERISTEMLESS（STM）的表达扩大。这一发现明确了 DG1 在调节向两侧对称过渡中的重要作用。此外，我们还发现 DG1 的这种调控作用可能并不取决于其在质体 RNA 编辑中的作用。然而，我们证明了 DG1 在建立双边对称中的功能是由 GENOMES UNCOUPLED 1 (GUN1) 基因介导的，后者抑制了 dg1-3 胚胎的过渡过程。总之，我们的研究结果揭示了 DG1 在功能上拮抗 GUN1，促进拟南芥胚胎的对称性从径向向两侧过渡，并突出了质体信号在植物胚胎发生过程中调控模式形成的作用。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.