Performance optimization of a DLD microfluidic device for separating deformable CTCs

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2024-08-14 DOI:10.1002/elps.202400136

Roya Mohammadali, Morteza Bayareh, Afshin Ahmadi Nadooshan

{"title":"Performance optimization of a DLD microfluidic device for separating deformable CTCs","authors":"Roya Mohammadali, Morteza Bayareh, Afshin Ahmadi Nadooshan","doi":"10.1002/elps.202400136","DOIUrl":null,"url":null,"abstract":"Deterministic lateral displacement (DLD) microfluidic devices work based on the streamlines created by an array of micro-posts. The configuration of pillars alters the isolation efficiency of these devices. The present paper optimizes the performance of a DLD device for isolating deformable circulating tumor cells. The input variables include cell diameter (d), Young's modulus (<math>\n <semantics>\n <msub>\n <mi>E</mi>\n <mi>s</mi>\n </msub>\n <annotation>${E}_s$</annotation>\n </semantics></math>), Reynolds number (Re), and tan θ, where θ is the tilted angle of micro-posts. The output, which is the response of the system, is DLD. The numerical simulation results are employed to optimize the device using the response surface method, leading to the proposition of a correlation to estimate DLD as a function of input variables. It is demonstrated that the maximum and minimum impacts on cell lateral displacement correspond to <math>\n <semantics>\n <msub>\n <mi>E</mi>\n <mi>s</mi>\n </msub>\n <annotation>${E}_s$</annotation>\n </semantics></math> and Re, respectively.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1775-1784"},"PeriodicalIF":3.0000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ELECTROPHORESIS","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/elps.202400136","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Deterministic lateral displacement (DLD) microfluidic devices work based on the streamlines created by an array of micro-posts. The configuration of pillars alters the isolation efficiency of these devices. The present paper optimizes the performance of a DLD device for isolating deformable circulating tumor cells. The input variables include cell diameter (d), Young's modulus ( $E_{s}$ ), Reynolds number (R_e), and tan θ, where θ is the tilted angle of micro-posts. The output, which is the response of the system, is DLD. The numerical simulation results are employed to optimize the device using the response surface method, leading to the proposition of a correlation to estimate DLD as a function of input variables. It is demonstrated that the maximum and minimum impacts on cell lateral displacement correspond to $E_{s}$ and R_e, respectively.

查看原文本刊更多论文

用于分离可变形 CTC 的 DLD 微流体设备的性能优化。

确定性横向位移（DLD）微流体设备的工作原理是微柱阵列产生的流线。支柱的配置会改变这些装置的隔离效率。本文优化了用于分离可变形循环肿瘤细胞的 DLD 设备的性能。输入变量包括细胞直径（d）、杨氏模量（E s ${E}_s$ ）、雷诺数（Re）和 tan θ，其中 θ 是微柱的倾斜角度。输出即系统响应，为 DLD。利用数值模拟结果，采用响应面法对设备进行优化，从而提出了一种相关方法，以估算 DLD 作为输入变量的函数。结果表明，对电池横向位移的最大和最小影响分别对应于 E s ${E}_s$ 和 Re。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.