Effect of photobiomodulation in an experimental in vitro model of asthma-Copd overlap

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2024-08-12 DOI:10.1002/jbio.202400124

Marina Bertoni Guerra, Kelly Gomes Santana, Marcos Momolli, Rodrigo Labat, Maria Cristina Chavantes, Stella Regina Zammuner, José Antonio Silva Júnior, Renata Kelly da Palma, Flavio Aimbire, Ana Paula Ligeiro de Oliveira

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引用次数: 0

Abstract

The objective of the study was to evaluate the effect of photobiomodulation (PBM) with laser on the inflammatory process in an experimental in vitro model of ACO. The groups were: (1) human bronchial epithelial cells (BEAS-2B); (2) BEAS-2B cells treated with dexamethasone; (3) BEAS-2B cells irradiated with laser; (4) BEAS-2B cells stimulated with cigarette smoke extract (CSE) + House Dust Mite (HDM); (5) BEAS-2B cells stimulated with CSE + HDM and treated with dexamethasone; (6) BEAS-2B cells incubated with CSE + HDM and irradiated with laser. After 24 h, cytokines were quantified. There was a reduction in TNF-α, IL-1β, IL-6, IL-4, IL-5, IL-13, IL-17, IL-21, IL-23, and an increase in IL-10 and IFN-γ in cells from the laser-irradiated ACO group compared to only ACO group. With these results, we can suggest that photobiomodulation acts in the modulation of inflammation observed in ACO, and may be a treatment option.

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光生物调节在哮喘-镉重叠实验体外模型中的效果。

该研究旨在评估激光光生物调节（PBM）对 ACO 实验性体外模型炎症过程的影响。实验组包括(1) 人支气管上皮细胞（BEAS-2B）；(2) 用地塞米松处理的 BEAS-2B 细胞；(3) 用激光照射的 BEAS-2B 细胞；(4) 用香烟烟雾提取物（CSE）+屋尘螨（HDM）刺激的 BEAS-2B 细胞；(5）用 CSE + HDM 刺激 BEAS-2B 细胞并用地塞米松处理；（6）用 CSE + HDM 培养 BEAS-2B 细胞并用激光照射。24 小时后，对细胞因子进行量化。与只用 ACO 组相比，用激光照射 ACO 组的细胞中 TNF-α、IL-1β、IL-6、IL-4、IL-5、IL-13、IL-17、IL-21、IL-23 减少，IL-10 和 IFN-γ 增加。根据这些结果，我们可以认为，光生物调制对 ACO 中观察到的炎症有调节作用，可能是一种治疗选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.