Microbiome and mycobiome analyses in continuous positive airway pressure devices.

IF 2.9 3区 医学 Q1 OTORHINOLARYNGOLOGY
Hyun Jin Min, Bo-Yun Choi, Woo Jun Sul, Hyung-Ju Cho
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引用次数: 0

Abstract

Objectives: Microorganisms are likely present in continuous positive airway pressure (CPAP) devices used daily. Considering the potential risk of infections among CPAP device users, here we aimed to compare the microbiomes in CPAP devices with those in nasal mucosal samples obtained from corresponding individuals using these devices.

Methods: We conducted a prospective cohort study at tertiary medical institutes. Samples were collected from the tubes and filters of CPAP devices and the nasal mucosa of corresponding individuals using these devices. Microbiomes and mycobiomes were analyzed using 16S ribosomal RNA and internal transcribed spacer region sequencing. Results were compared according to the sampling site and usage duration for each patient.

Results: Overall, 27 paired human nasal mucosa and CPAP samples were analyzed. Bacteria were present in 7 of 27 tubes (29.6%) and 22 of 27 filters (81.5%). Fungi were present in 2 of the 27 tubes (7.4%) and 16 of the 27 filters (59.3%). Actinobacteria and Firmicutes were the predominant phyla among all samples. Fungi were not detected in any of the nasal mucosal samples. However, Basidiomycota and Ascomycota were predominant in the CPAP filters and tube samples. No significant associations were identified among the results according to sampling site and usage duration.

Conclusion: Bacteria or fungi can be detected to some extent in CPAP samples even if the CPAP usage period is short. The association between respiratory infections and these microbiomes or mycobiomes was not investigated. Further research might be required to determine the risk posed by CPAP devices as a microbial contamination source.

持续气道正压装置中的微生物组和霉菌生物组分析。
目的:日常使用的持续气道正压(CPAP)装置中可能存在微生物。考虑到 CPAP 设备使用者的潜在感染风险,我们在此旨在比较 CPAP 设备中的微生物群与从使用这些设备的相应个体中获得的鼻粘膜样本中的微生物群:方法:我们在三级医疗机构开展了一项前瞻性队列研究。方法:我们在三级医疗机构开展了一项前瞻性队列研究,从 CPAP 设备的管道和过滤器以及使用这些设备的相应患者的鼻腔粘膜中采集样本。使用 16S 核糖体 RNA 和内部转录间隔区测序分析微生物组和霉菌生物组。根据每位患者的采样部位和使用时间对结果进行比较:结果:共分析了 27 份成对的人类鼻粘膜和 CPAP 样本。27 个管道中有 7 个存在细菌(29.6%),27 个过滤器中有 22 个存在细菌(81.5%)。真菌存在于 27 个试管中的 2 个(7.4%)和 27 个过滤器中的 16 个(59.3%)。放线菌和真菌是所有样本中的主要菌门。在所有鼻粘膜样本中均未检测到真菌。不过,在 CPAP 过滤器和管道样本中,主要是基枝菌纲和子囊菌纲。根据采样部位和使用时间的不同,结果之间没有发现明显的关联:结论:即使使用 CPAP 的时间较短,也能在 CPAP 样本中检测到一定程度的细菌或真菌。呼吸道感染与这些微生物群或真菌生物群之间的关系尚未得到研究。要确定 CPAP 设备作为微生物污染源所带来的风险,可能还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.90
自引率
6.70%
发文量
49
审稿时长
6-12 weeks
期刊介绍: Clinical and Experimental Otorhinolaryngology (Clin Exp Otorhinolaryngol, CEO) is an international peer-reviewed journal on recent developments in diagnosis and treatment of otorhinolaryngology-head and neck surgery and dedicated to the advancement of patient care in ear, nose, throat, head, and neck disorders. This journal publishes original articles relating to both clinical and basic researches, reviews, and clinical trials, encompassing the whole topics of otorhinolaryngology-head and neck surgery. CEO was first issued in 2008 and this journal is published in English four times (the last day of February, May, August, and November) per year by the Korean Society of Otorhinolaryngology-Head and Neck Surgery. The Journal aims at publishing evidence-based, scientifically written articles from different disciplines of otorhinolaryngology field. The readership contains clinical/basic research into current practice in otorhinolaryngology, audiology, speech pathology, head and neck oncology, plastic and reconstructive surgery. The readers are otolaryngologists, head and neck surgeons and oncologists, audiologists, and speech pathologists.
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