Malvidin From Malva sylvestris L. Ameliorates Allergic Responses in Ovalbumin-Induced Allergic Rhinitis Mouse Model via the STAT6/GATA3 Pathway.

IF 2.5 3区医学 Q1 OTORHINOLARYNGOLOGY

American Journal of Rhinology & Allergy Pub Date : 2024-11-01 Epub Date: 2024-08-12 DOI:10.1177/19458924241272944

Luo Yirui, Li Tao, Liu Ruowu, Zhou Jiao, Zhou Jing, Xu Xiaodong, Yan Yan, Claus Bachert, Du Jintao, Ba Luo

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引用次数: 0

Abstract

Background: Malva sylvestris L. (commonly known as mallow) has been widely used in traditional Tibetan formulations to treat allergic rhinitis (AR), and malvidin is a key anti-inflammation constituent of this plant.

Objective: The present study aimed to evaluate the potential therapeutic effect and mechanism of malvidin in an AR mouse model.

Methods: Malvidin's efficacy was evaluated in an AR mouse model induced by ovalbumin (OVA) sensitization and challenge. The factors, such as nasal symptoms, serum OVA-specific immunoglobulin E (IgE) levels, histological changes in the nasal mucosa, and expressions of Th1, Th2, Th17, and Tregs and their cytokines, were assessed. Western blotting was used to analyze the effect of malvidin on signal transducer and activator of transcription 6 (STAT6) and GATA3 expression levels.

Results: Malvidin reduced the allergic symptoms and serum levels of OVA-specific IgE in the AR model. Histological analysis indicated that malvidin alleviates nasal mucosal edema, eosinophil infiltration, and goblet cell proliferation. In addition, it altered the expression of Th1/Th2/Th17-related cytokines, enhanced the Treg population, and reduced Th2-mediated immunity by suppressing the phosphorylation of STAT6 and expression of the GATA3 protein.

Conclusions: Malvidin significantly improved allergic symptoms in an OVA-induced AR mouse model by modulating Th1/Th2 immune responses and suppressing the STAT6/GATA3 pathway, indicating its potential as a naturally sourced agent for AR management.

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通过 STAT6/GATA3 通路改善卵清蛋白诱导的过敏性鼻炎小鼠模型的过敏反应

背景：Malva sylvestris L.（俗称锦葵）在藏族传统配方中被广泛用于治疗过敏性鼻炎（AR），而麦冬苷是该植物的一种主要抗炎成分：本研究旨在评估麦饭石苷在 AR 小鼠模型中的潜在治疗效果和机制：方法：在由卵清蛋白（OVA）致敏和挑战诱导的AR小鼠模型中评估麦冬苷的疗效。评估因素包括鼻部症状、血清 OVA 特异性免疫球蛋白 E (IgE) 水平、鼻黏膜组织学变化以及 Th1、Th2、Th17 和 Tregs 及其细胞因子的表达。用 Western 印迹法分析了麦饭石对信号转导和转录激活因子 6（STAT6）和 GATA3 表达水平的影响：结果：在AR模型中，麦饭石可减轻过敏症状和血清中OVA特异性IgE的水平。组织学分析表明，麦饭石可减轻鼻粘膜水肿、嗜酸性粒细胞浸润和上睑细胞增殖。此外，它还能改变 Th1/Th2/Th17 相关细胞因子的表达，增强 Treg 群体，并通过抑制 STAT6 的磷酸化和 GATA3 蛋白的表达来降低 Th2 介导的免疫力：结论：麦饭石通过调节Th1/Th2免疫反应和抑制STAT6/GATA3通路，明显改善了OVA诱导的AR小鼠模型的过敏症状，这表明麦饭石具有作为天然药物治疗AR的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Rhinology & Allergy 医学-耳鼻喉科学

CiteScore

5.60

自引率

11.50%

发文量

审稿时长

4-8 weeks

期刊介绍： The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.