Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2024-08-13 DOI:10.1007/s10735-024-10246-w

Junpei Zhang, Hongyun Zhang, Ying Chen, Shiyao Chen, Hailing Liu

{"title":"Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway","authors":"Junpei Zhang, Hongyun Zhang, Ying Chen, Shiyao Chen, Hailing Liu","doi":"10.1007/s10735-024-10246-w","DOIUrl":null,"url":null,"abstract":"<div><p>Dendrobine (DDB), an alkaloid isolated from the Chinese herb Dendrobium, has antioxidant and anti-inflammatory effects; however, whether DDB reduces oleic acid (OA)-induced lipid accumulation remains unclear. OA-induced lipid accumulation model of HepG2 cells were treated with DDB. Cellular lipid deposition was assessed by Oil Red O (ORO) staining and triglyceride and total cholesterol detection. RNA-Sequencing (RNA-seq), biological function analysis, and transcription factor (TFs) prediction were combined to identify key TF in the DDB-treated OA model. Finally, the roles of FOS and METTL14 were examined using a DDB-induced lipid accumulation model. DDB inhibited OA-induced lipid accumulation. We identified 895 differentially expressed genes (DEGs) that were mainly enriched in various biological processes of lipid synthesis and transport. Four transcription factors (SOX9, MLXIPL, FOS, and JUN) associated with lipid metabolism and FOS levels in the OA-induced lipid accumulation model after DDB treatment had the greatest changes in expression change. Overexpression of FOS alleviates the inhibitory effect of DDB on OA-induced lipid accumulation. METTL14 is a target gene of FOS, and simultaneous interference with METTL14 in cells with high FOS expression restored the alleviating effect of DDB on lipid accumulation. DDB alleviated OA-induced lipid accumulation by inhibiting the FOS/METTL14 pathway.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Histology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10735-024-10246-w","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Dendrobine (DDB), an alkaloid isolated from the Chinese herb Dendrobium, has antioxidant and anti-inflammatory effects; however, whether DDB reduces oleic acid (OA)-induced lipid accumulation remains unclear. OA-induced lipid accumulation model of HepG2 cells were treated with DDB. Cellular lipid deposition was assessed by Oil Red O (ORO) staining and triglyceride and total cholesterol detection. RNA-Sequencing (RNA-seq), biological function analysis, and transcription factor (TFs) prediction were combined to identify key TF in the DDB-treated OA model. Finally, the roles of FOS and METTL14 were examined using a DDB-induced lipid accumulation model. DDB inhibited OA-induced lipid accumulation. We identified 895 differentially expressed genes (DEGs) that were mainly enriched in various biological processes of lipid synthesis and transport. Four transcription factors (SOX9, MLXIPL, FOS, and JUN) associated with lipid metabolism and FOS levels in the OA-induced lipid accumulation model after DDB treatment had the greatest changes in expression change. Overexpression of FOS alleviates the inhibitory effect of DDB on OA-induced lipid accumulation. METTL14 is a target gene of FOS, and simultaneous interference with METTL14 in cells with high FOS expression restored the alleviating effect of DDB on lipid accumulation. DDB alleviated OA-induced lipid accumulation by inhibiting the FOS/METTL14 pathway.

Abstract Image

查看原文本刊更多论文

石斛碱通过抑制 FOS/METTL14 通路缓解油酸诱导的脂质积累。

铁皮石斛碱（DDB）是从中草药铁皮石斛中分离出来的一种生物碱，具有抗氧化和抗炎作用；然而，DDB 是否能减少油酸（OA）诱导的脂质积累仍不清楚。用 DDB 处理 OA 诱导的脂质积累模型 HepG2 细胞。细胞脂质沉积通过油红 O（ORO）染色和甘油三酯及总胆固醇检测进行评估。结合RNA测序（RNA-seq）、生物功能分析和转录因子（TFs）预测，确定了DDB处理的OA模型中的关键TF。最后，利用DDB诱导的脂质积累模型检验了FOS和METTL14的作用。DDB抑制了OA诱导的脂质积累。我们发现了895个差异表达基因（DEGs），它们主要富集于脂质合成和运输的各种生物学过程中。在DDB处理后的OA诱导脂质积累模型中，与脂质代谢和FOS水平相关的四个转录因子（SOX9、MLXIPL、FOS和JUN）的表达变化最大。过表达 FOS 可减轻 DDB 对 OA 诱导的脂质积累的抑制作用。METTL14是FOS的靶基因，在FOS高表达的细胞中同时干扰METTL14，可恢复DDB对脂质积累的缓解作用。DDB通过抑制FOS/METTL14途径缓解了OA诱导的脂质积累。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.