Autophagic genes and antioxidant status during doxorubicin therapy under temperature-stressed conditions in breast cancer cells

IF 1 Q4 GENETICS & HEREDITY
Firas S. Salah , Amer T. Tawfeeq , Noah A. Mahmood , Khansaa R. Al-Saadi , Tiba H. Jaafar , Amna Y. Al-Obaidi
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引用次数: 0

Abstract

Objectives

Autophagy as a cellular event swings between apoptosis induction and cellular maintenance during chemotherapy. The interplay between autophagy and reactive oxygen species (ROS) remains obscure in cancer progression and treatment. This study aimed to determine the combination effect of chemotherapy and hyperthermia on cancer cell proliferation, autophagy signaling, and oxidative stress status.

Methods

To estimate the autophagic genes' role and antioxidant capacity involvement during chemotherapy, human breast cancer cell lines (MCF7 and CAL51) were exposed to a high temperature at 42 °C and treated with doxorubicin (DOX). The MTT assays were performed to determine the cell survival levels, and the autophagic gene expression levels (ATG5, LC3A, LC3B, and Beclin1) were analyzed. The intracellular TAS (total antioxidant status) and SOD (superoxide dismutase) activities were determined.

Results

The combined effects of high temperature with chemotherapy significantly reduced cell viability and cell survival compared to cells treated with the chemotherapeutic agent DOX alone. The cell's autophagic gene activities were significantly increased after exposure to 42 °C temperature and DOX-based chemotherapy compared to the cells treated with DOX alone. It was observed that TAS and SOD activities were increased in the cell lines exposed to high temperatures with DOX compared to the cells treated with DOX alone. Increased autophagic gene expression levels and cell death were observed in response to the high temperature and DOX treatment in breast cancer cells.

Conclusion

The study may provide a plausible route and treatment plan for the individual adoption of cancer chemotherapy and be developed as part of the personalized medicine protocol.

乳腺癌细胞在温度应激条件下接受多柔比星治疗期间的自噬基因和抗氧化状态
目的自噬是化疗过程中在诱导细胞凋亡和维持细胞活力之间摇摆的细胞事件。在癌症进展和治疗过程中,自噬与活性氧(ROS)之间的相互作用仍然模糊不清。本研究旨在确定化疗和高热对癌细胞增殖、自噬信号转导和氧化应激状态的联合影响。方法为了评估自噬基因在化疗过程中的作用和抗氧化能力,将人类乳腺癌细胞系(MCF7 和 CAL51)暴露于 42 ℃ 的高温下,并用多柔比星(DOX)处理。通过 MTT 检测确定细胞存活水平,并分析自噬基因(ATG5、LC3A、LC3B 和 Beclin1)的表达水平。结果与单用化疗药物 DOX 处理的细胞相比,高温与化疗的联合作用显著降低了细胞存活率和细胞存活率。与单独使用 DOX 的细胞相比,暴露于 42 °C 温度和 DOX 化疗后细胞的自噬基因活性明显增加。据观察,与单独使用 DOX 的细胞相比,暴露于高温和 DOX 的细胞系中的 TAS 和 SOD 活性均有所提高。结论该研究可为个体采用癌症化疗提供合理的途径和治疗方案,并可作为个性化医疗方案的一部分加以开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Gene Reports
Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.30
自引率
7.70%
发文量
246
审稿时长
49 days
期刊介绍: Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.
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